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JXB Advance Access originally published online on May 3, 2008
Journal of Experimental Botany 2008 59(8):1973-1986; doi:10.1093/jxb/ern069
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Identification of woolliness response genes in peach fruit after post-harvest treatments

Mauricio González-Agüero1 *, Leonardo Pavez1, Freddy Ibáñez1, Igor Pacheco1, Reinaldo Campos-Vargas2, Lee A. Meisel3, Ariel Orellana3, Julio Retamales4, Herman Silva3, Mauricio González1 and Verónica Cambiazo1,{dagger}

1Laboratorio de Bioinformática y Expresión Génica, INTA-Universidad de Chile, Millennium Nucleus Center for Genomics of the Cell (CGC), Santiago, Chile
2Institute of Agricultural Research (INIA-La Platina), Santiago, Chile
3Millennium Nucleus in Plant Cell Biology and Plant Biotechnology Center, Andres Bello University, Santiago, Chile
4Faculty of Agricultural Sciences, Universidad de Chile, Santiago, Chile

{dagger} To whom correspondence should be addressed. E-mail: vcambiaz{at}inta.cl

Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. This disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. However, knowledge about the molecular basis of peach woolliness is still incomplete. To address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from a ripe peach fruit cDNA library was developed and used. Gene expression changes of peach fruit (Prunus persica cv. O'Henry) ripened for 7 d at 21 °C (juicy fruit) were compared with those of fruit stored for 15 d at 4 °C and then ripened for 7 d at 21 °C (woolly fruit). A total of 106 genes were found to be differentially expressed between juicy and woolly fruit. Data analysis indicated that the activity of most of these genes (>90%) was repressed in the woolly fruit. In cold-stored peaches (cv. O'Henry), the expression level of selected genes (cobra, endopolygalacturonase, cinnamoyl-CoA-reductase, and rab11) was lower than in the juicy fruit, and it remained low in woolly peaches after ripening, a pattern that was conserved in woolly fruit from two other commercial cultivars (cv. Flamekist and cv. Elegant Lady). In addition, the results of this study indicate that molecular changes during fruit woolliness involve changes in the expression of genes associated with cell wall metabolism and endomembrane trafficking. Overall, the results reported here provide an initial characterization of the transcriptome activity of peach fruit under different post-harvest treatments.

Key words: Cell wall, endomembrane traffic, gene expression, peach, woolly fruit

* Present address: Institute of Agricultural Research (INIA-La Platina), Santiago, Chile.

Received 15 October 2007; Revised 28 January 2008 Accepted 31 January 2008


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