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JXB Advance Access published online on April 23, 2008
Journal of Experimental Botany, doi:10.1093/jxb/ern090
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Is internucleosomal DNA fragmentation an indicator of programmed death in plant cells?

Andrea Kuthanova, Zdenek Opatrny and Lukas Fischer*

Charles University in Prague, Faculty of Science, Department of Plant Physiology, Vinicna 5, CZ 128 44 Prague 2, Czech Republic

* To whom correspondence should be addressed. E-mail: lukasf{at}natur.cuni.cz

Specific DNA fragmentation into oligonucleosomal units occurs during programmed cell death (PCD) in both animal and plant cells, usually being regarded as an indicator of its apoptotic character. This internucleosomal DNA fragmentation is demonstrated in tobacco suspension and leaf cells, which were killed immediately by freezing in liquid nitrogen, and homogenization or treatment with Triton X-100. Although these cells could not activate and realize the respective enzymatic processes in a programmed manner, the character of DNA fragmentation was similar to that in the cells undergoing typical gradual PCD induced by 50 µM CdSO4. This internucleosomal DNA fragmentation was connected with the action of cysteine proteases and the loss of membrane, in particular tonoplast, integrity. The mechanisms of DNase activation in the rapidly killed cells, hypothetical biological relevance, and implications for the classification of cell death are discussed.

Key words: Non-PCD internucleosomal DNA fragmentation, programmed cell death, protease inhibitor, tobacco BY-2 cell line, vacuolar integrity

Received 8 January 2008; Revised 3 March 2008 Accepted 4 March 2008


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