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JXB Advance Access originally published online on May 2, 2008
Journal of Experimental Botany 2008 59(8):2267-2276; doi:10.1093/jxb/ern097
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Rice–arsenate interactions in hydroponics: whole genome transcriptional analysis

Gareth J. Norton*, Daniel E. Lou-Hing, Andrew A. Meharg and Adam H. Price

Department of Plant and Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 3UU, UK

* To whom correspondence should be addressed. E-mail: g.norton{at}abdn.ac.uk

Rice (Oryza sativa) varieties that are arsenate-tolerant (Bala) and -sensitive (Azucena) were used to conduct a transcriptome analysis of the response of rice seedlings to sodium arsenate (AsV) in hydroponic solution. RNA extracted from the roots of three replicate experiments of plants grown for 1 week in phosphate-free nutrient with or without 13.3 µM AsV was used to challenge the Affymetrix (52K) GeneChip Rice Genome array. A total of 576 probe sets were significantly up-regulated at least 2-fold in both varieties, whereas 622 were down-regulated. Ontological classification is presented. As expected, a large number of transcription factors, stress proteins, and transporters demonstrated differential expression. Striking is the lack of response of classic oxidative stress-responsive genes or phytochelatin synthases/synthatases. However, the large number of responses from genes involved in glutathione synthesis, metabolism, and transport suggests that glutathione conjugation and arsenate methylation may be important biochemical responses to arsenate challenge. In this report, no attempt is made to dissect differences in the response of the tolerant and sensitive variety, but analysis in a companion article will link gene expression to the known tolerance loci available in the BalaxAzucena mapping population.

Key words: Arsenate tolerance, candidate genes, glutathione-S-transferase, microarray, Oryza sativa

Received 7 December 2007; Revised 5 February 2008 Accepted 7 March 2008


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