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JXB Advance Access originally published online on July 9, 2008
Journal of Experimental Botany 2008 59(11):3169-3185; doi:10.1093/jxb/ern172
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Genetic and molecular characterization of three novel S-haplotypes in sour cherry (Prunus cerasus L.)

Tatsuya Tsukamoto1 *, Daniel Potter2, Ryutaro Tao3, Cristina P. Vieira4, Jorge Vieira4 and Amy F. Iezzoni1,{dagger}

1Department of Horticulture, Michigan State University, East Lansing, MI 48824, USA
2Department of Plant Sciences, University of California, Davis, CA 95616–8780, USA
3Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
4Instituto de Biologia Molecular e Celular, University of Porto, 4150–180 Porto, Portugal

{dagger} To whom correspondence should be addressed. E-mail: iezzoni{at}msu.edu

Tetraploid sour cherry (Prunus cerasus L.) exhibits gametophytic self-incompatibility (GSI) whereby the specificity of self-pollen rejection is controlled by alleles of the stylar and pollen specificity genes, S-RNase and SFB (S haplotype-specific F-box protein gene), respectively. As sour cherry selections can be either self-compatible (SC) or self-incompatible (SI), polyploidy per se does not result in SC. Instead the genotype-dependent loss of SI in sour cherry is due to the accumulation of non-functional S-haplotypes. The presence of two or more non-functional S-haplotypes within sour cherry 2x pollen renders that pollen SC. Two new S-haplotypes from sour cherry, S33 and S34, that are presumed to be contributed by the P. fruticosa species parent, the complete S-RNase and SFB sequences of a third S-haplotype, S35, plus the presence of two previously identified sweet cherry S-haplotypes, S14 and S16 are described here. Genetic segregation data demonstrated that the S16-, S33-, S34-, and S35-haplotypes present in sour cherry are fully functional. This result is consistent with our previous finding that ‘hetero-allelic’ pollen is incompatible in sour cherry. Phylogenetic analyses of the SFB and S-RNase sequences from available Prunus species reveal that the relationships among S-haplotypes show no correspondence to known organismal relationships at any taxonomic level within Prunus, indicating that polymorphisms at the S-locus have been maintained throughout the evolution of the genus. Furthermore, the phylogenetic relationships among SFB sequences are generally incongruent with those among S-RNase sequences for the same S-haplotypes. Hypotheses compatible with these results are discussed.

Key words: Prunus cerasus, self-incompatibility, SFB, S-RNase

* Present address: Department of Plant Sciences, University of Arizona, Tucson, AZ85721, USA.

Received 20 March 2008; Revised 22 May 2008 Accepted 27 May 2008


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