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JXB Advance Access published online on August 1, 2008
Journal of Experimental Botany, doi:10.1093/jxb/ern196
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Gene expression and sensitivity in response to copper stress in rice leaves*

Emi Sudo1, Misao Itouga1, Kayo Yoshida-Hatanaka1, Yoshiro Ono2 and Hitoshi Sakakibara1,{dagger}

1RIKEN Plant Science Center, Suehiro 1-7-22, Tsurumi, Yokohama 230-0045, Japan
2Department of Environmental and Civil Engineering, Faculty of Environmental Science and Technology, Okayama University, 3-1-1, Tsushima-naka, Okayama-shi, Okayama 700-8530, Japan

{dagger} To whom correspondence should be addressed. E-mail: sakaki{at}riken.jp

Gene expression in response to Cu stress in rice leaves was quantified using DNA microarray (Agilent 22K Rice Oligo Microarray) and real-time PCR technology. Rice plants were grown in hydroponic solutions containing 0.3 (control), 10, 45, or 130 µM of CuCl2, and Cu accumulation and photosynthesis inhibition were observed in leaves within 1 d of the start of treatment. Microarray analysis flagged 305 Cu-responsive genes, and their expression profile showed that a large proportion of general and defence stress response genes are up-regulated under excess Cu conditions, whereas photosynthesis and transport-related genes are down-regulated. The Cu sensitivity of each Cu-responsive gene was estimated by the median effective concentration value (EC50) and the range of fold-changes (F) under the highest (130 µM) Cu conditions (|log2F|130). Our results indicate that defence-related genes involved in phytoalexin and lignin biosynthesis were the most sensitive to Cu, and that plant management of abiotic and pathogen stresses has overlapping components, possibly including signal transduction.

Key words: Copper-sensitivity, DNA microarray, excess copper stress, gene expression, Oryza sativa L

* Microarray data have been deposited in The National Center for Biotechnology Information Gene Expression Omnibus (NCBI GEO) database under accession number GSE11021 [NCBI GEO] .

Received 7 April 2008; Revised 24 June 2008 Accepted 2 July 2008


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