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JXB Advance Access published online on October 1, 2008
Journal of Experimental Botany, doi:10.1093/jxb/ern235
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Exposure to nitric oxide protects against oxidative damage but increases the labile iron pool in sorghum embryonic axes

Sebastián Jasid, Marcela Simontacchi and Susana Puntarulo*

Physical Chemistry-PRALIB, School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, Buenos Aires, C1113AAD, Argentina

* To whom correspondence should be addressed: E-mail: susanap{at}ffyb.uba.ar

Sodium nitroprusside (SNP) and diethylenetriamine NONOate (DETA NONOate), were used as the source of exogenous NO to study the effect of NO upon germination of sorghum (Sorghum bicolor (L.) Moench) seeds through its possible interaction with iron. Modulation of cellular Fe status could be an important factor for the establishment of oxidative stress and the regulation of plant physiology. Fresh and dry weights of the embryonic axes were significantly increased in the presence of 0.1 mM SNP, as compared to control. Spin trapping EPR was used to assess the NO content in axes from control seeds after 24 h of imbibition (2.4±0.2 nmol NO g–1 FW) and seeds exposed to 0.01, 0.1, and 1 mM SNP (3.1±0.3, 4.6±0.2, and 6.0±0.9 nmol NO g–1 FW, respectively) and 1 mM DETA NONOate (6.2±0.6 nmol NO g–1 FW). Incubation of seeds with 1 mM SNP protected against oxidative damage to lipids and maintained membrane integrity. The content of the deferoxamine–Fe (III) complex significantly increased in homogenates of axes excised from seeds incubated in the presence of 1 mM SNP or 1 mM DETA NONOate as compared to the control (19±2 nmol Fe g–1 FW, 15.2±0.5 nmol Fe g–1 FW, and 8±1 nmol Fe g–1 FW, respectively), whereas total Fe content in the axes was not affected by the NO donor exposure. Data presented here provide experimental evidence to support the hypothesis that increased availability of NO drives not only protective effects to biomacromolecules, but to increasing the Fe availability for promoting cellular development as well.

Key words: Iron, labile iron pool, nitric oxide, protein nitration, protein oxidation, sorghum

Received 29 May 2008; Revised 19 August 2008 Accepted 26 August 2008


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