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JXB Advance Access first published online on May 19, 2009
This version published online on May 26, 2009
Journal of Experimental Botany, doi:10.1093/jxb/erp153
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© The Author [2009]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

AtRAB-H1b and AtRAB-H1c GTPases, homologues of the yeast Ypt6, target reporter proteins to the Golgi when expressed in Nicotiana tabacum and Arabidopsis thaliana

Jorunn Nergaard Johansen1 *, Cheung-Ming Chow2 {dagger}, Ian Moore2 and Chris Hawes1,{ddagger}

1School of Life Sciences, Oxford Brookes University, Gipsy Lane, Oxford OX3 0BP, UK
2Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK

{ddagger} To whom correspondence should be addressed: E-mail: chawes{at}brookes.ac.uk

Ypt/Rab GTPases act as key regulators of intracellular traffic through the conformational differences exhibited by their GTP or GDP-bound forms. In this paper, two Arabidopsis Ypt6 homologues, AtRAB-H1b and AtRAB-H1c were characterized and compared. Using a live cell imaging approach, it is shown that yellow fluorescent protein-fusions (YFP) of AtRAB-H1b and AtRAB-H1c locate to the Golgi and to the cytosol in both Nicotiana tabacum and in Arabidopsis thaliana. In addition, YFP-AtRAB-H1b targets an as yet unknown compartment not labelled by YFP-AtRAB-H1c or Golgi markers. It is also shown that the subcellular location of YFP-AtRAB-H1b and YFP-AtRAB-H1c is affected by the state of GTP-binding and that expression of a GTP-deficient mutant results in increased apoplastic fluorescence of a secretory form of YFP.

Key words: Confocal microscopy, GFP, Golgi apparatus, Rab GTPase

* Present address: Institute for Cancer Research, Department of Immunology, Rikshospitalet-Radiumhospitalet Medical Centre, Montebello 0310 Oslo, Norway.

{dagger} Department of Biology, Science Centre, The Chinese University of Hong Kong, Shatin, Hong Kong SAR.

The incorrect article type heading ‘Review Paper’ was displayed in the original version of this paper. This has now been changed to ‘Research Paper’

Received 16 December 2008; Revised 17 April 2009 Accepted 20 April 2009


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