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JXB Advance Access published online on June 23, 2009
Journal of Experimental Botany, doi:10.1093/jxb/erp191
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Further characterization of ferric—phytosiderophore transporters ZmYS1 and HvYS1 in maize and barley

Daisei Ueno, Naoki Yamaji and Jian Feng Ma*

Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan

* To whom correspondence should be addressed. E-mail: maj{at}rib.okayama-u.ac.jp

Roots of some gramineous plants secrete phytosiderophores in response to iron deficiency and take up Fe as a ferric–phytosiderophore complex through the transporter YS1 (Yellow Stripe 1). Here, this transporter in maize (ZmYS1) and barley (HvYS1) was further characterized and compared in terms of expression pattern, diurnal change, and tissue-type specificity of localization. The expression of HvYS1 was specifically induced by Fe deficiency only in barley roots, and increased with the progress of Fe deficiency, whereas ZmYS1 was expressed in maize in the leaf blades and sheaths, crown, and seminal roots, but not in the hypocotyl. HvYS1 expression was not induced by any other metal deficiency. Furthermore, in maize leaf blades, the expression was higher in the young leaf blades showing severe chlorosis than in the old leaf blades showing no chlorosis. The expression of HvYS1 showed a distinct diurnal rhythm, reaching a maximum before the onset of phytosiderophore secretion. In contrast, ZmYS1 did not show such a rhythm in expression. Immunostaining showed that ZmYS1 was localized in the epidermal cells of both crown and lateral roots, with a polar localization at the distal side of the epidermal cells. In maize leaves, ZmYS1 was localized in mesophyll cells, but not epidermal cells. These differences in gene expression pattern and tissue-type specificity of localization suggest that HvYS1 is only involved in primary Fe acquisition by barley roots, whereas ZmYS1 is involved in both primary Fe acquisition and intracellular transport of iron and other metals in maize.

Key words: Barley, expression pattern, iron, localization, maize, phytosiderophore, transporter

Received 2 February 2009; Revised 23 April 2009 Accepted 18 May 2009


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