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JXB Advance Access published online on October 8, 2009
Journal of Experimental Botany, doi:10.1093/jxb/erp296
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Hypoxia-responsive microRNAs and trans-acting small interfering RNAs in Arabidopsis

Dov Moldovan1,2, Andrew Spriggs1, Jun Yang1, Barry J. Pogson2, Elizabeth S. Dennis1 and Iain W. Wilson1,*

1CSIRO Plant Industry, GPO Box 1600, Canberra ACT 2601, Australia
2ARC Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Canberra, Australia

* To whom correspondence should be addressed: E-mail: iain.wilson{at}csiro.au

Low-oxygen (hypoxia) stress associated with natural phenomena such as waterlogging, results in widespread transcriptome changes and a metabolic switch from aerobic respiration to anaerobic fermentation. High-throughput sequencing of small RNA libraries obtained from hypoxia-treated and control root tissue identified a total of 65 unique microRNA (miRNA) sequences from 46 families, and 14 trans-acting small interfering RNA (tasiRNA) from three families. Hypoxia resulted in changes to the abundance of 46 miRNAs from 19 families, and all three tasiRNA families. Chemical inhibition of mitochondrial respiration caused similar changes in expression in a majority of the hypoxia-responsive small RNAs analysed. Our data indicate that miRNAs and tasiRNAs play a role in gene regulation and possibly developmental responses to hypoxia, and that a major signal for these responses is likely to be dependent on mitochondrial function.

Key words: Abiotic stress, Arabidopsis thaliana, deep sequencing, hypoxia, microRNA, mitochondria, trans-acting small interfering RNA

Received 20 July 2009; Revised 3 September 2009 Accepted 7 September 2009


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