JXB Advance Access published online on October 23, 2009
Journal of Experimental Botany, doi:10.1093/jxb/erp307
© 2009 The Author(s).
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RESEARCH PAPER |
Flower numbers, pod production, pollen viability, and pistil function are reduced and flower and pod abortion increased in chickpea (Cicer arietinum L.) under terminal drought
1Centre for Legumes in Mediterranean Agriculture, M080, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
2MOE Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou, Gansu Province 730000, PR China
3School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
4Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
* To whom correspondence should be addressed. E-mail: fangxw{at}lzu.edu.cn
Terminal drought during the reproductive stage is a major constraint to yield of chickpea in many regions of the world. Termination of watering (WS) during podding in a small-seeded desi chickpea (Cicer arietinum L.) cultivar, Rupali, and a large-seeded kabuli chickpea cultivar, Almaz, induced a decrease in predawn leaf water potential (LWP), in the rate of photosynthesis, and in stomatal conductance. Compared to well-watered (WW) controls, the WS treatment reduced flower production by about two-thirds. In the WW treatment, about 15% of the flowers aborted and 42% (Rupali) and 67% (Almaz) of the pods aborted, whereas in the WS treatment 37% and 56% of the flowers aborted and 54% and 73% of the pods aborted, resulting in seed yields of 33% and 15% of the yields in WW plants in Rupali and Almaz, respectively. In vitro pollen viability and germination in Rupali decreased by 50% and 89% in the WS treatment, and pollen germination decreased by 80% in vivo when pollen from a WS plant was placed on a stigma of a WW plant. While about 37% of the germinated pollen tubes from WW plants and 22% from the WS plants reached the ovary in the WW plants, less than 3% of pollen grains reached the ovary when pollen from either WS or WW plants was placed on a stigma of a WS plant. It is concluded that, in addition to pod abortion, flower abortion is an important factor limiting yield in chickpea exposed to terminal drought and that water deficit impaired the function of the pistil/style more than the pollen.
Key words: Flower production, pistil, pod set, pollen, seed yield, stigma, style
Received 22 June 2009; Revised 13 September 2009 Accepted 21 September 2009