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JXB Advance Access published online on October 23, 2009
Journal of Experimental Botany, doi:10.1093/jxb/erp316
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers

Filomena Giorno1,*, Mieke Wolters-Arts1, Stefania Grillo2, Klaus-Dieter Scharf3, Wim H. Vriezen1 and Celestina Mariani1

1Department of Plant Cell Biology, IWWR, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands
2CNR-IGV Institute of Plant Genetics, Via Università 133, 80055 Portici, Naples, Italy
3Molecular Cell Biology, Goethe University, Max-von-Laue-Str. 9, D-60438 Frankfurt/M., Germany

* To whom correspondence should be adddressed. E-mail: mgiorno{at}science.ru.nl

The high sensitivity of male reproductive cells to high temperatures may be due to an inadequate heat stress response. The results of a comprehensive expression analysis of HsfA2 and Hsp17-CII, two important members of the heat stress system, in the developing anthers of a heat-tolerant tomato genotype are reported here. A transcriptional analysis at different developmental anther/pollen stages was performed using semi-quantitative and real-time PCR. The messengers were localized using in situ RNA hybridization, and protein accumulation was monitored using immunoblot analysis. Based on the analysis of the gene and protein expression profiles, HsfA2 and Hsp17-CII are finely regulated during anther development and are further induced under both short and prolonged heat stress conditions. These data suggest that HsfA2 may be directly involved in the activation of protection mechanisms in the tomato anther during heat stress and, thereby, may contribute to tomato fruit set under adverse temperatures.

Key words: Anther development, heat stress, HsfA2, Hsp17-CII, pollen, tomato

{dagger} Present address: Nunhems Netherlands BV, PO Box 4005, 6080 AA Haelen, The Netherlands

Received 5 August 2009; Revised 6 October 2009 Accepted 8 October 2009


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