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JXB Advance Access published online on October 30, 2009
Journal of Experimental Botany, doi:10.1093/jxb/erp319
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Light can rescue auxin-dependent synchrony of cell division in a tobacco cell line

Fei Qiao1,*, Jan Petrásek2,3 and Peter Nick1

1Institute of Botany 1, University of Karlsruhe, Kaiserstrasse 2, D-76128 Karlsruhe, Germany
2Institute of Experimental Botany, Czech Academy of Science, Rozvojová 263, 16502 Prague 6, Czech Republic
3Department of Plant Physiology, Faculty of Science, Charles University, Vinicná 5, 12844 Prague 2, Czech Republic

* To whom correspondence should be addressed. E-mail: fei.qiao{at}bio.uni-karlsruhe.de

Pattern formation in plants has to cope with ambient variability and therefore must integrate environmental cues such as light. Synchrony of cell divisions was previously observed in cell files of tobacco suspension cultures, which represents a simple case of pattern formation. To develop cellular approaches for light-dependent patterning, light-responsive tobacco cell lines were screened from the cell line Nicotiana tabacum L. cv. Virginia Bright Italia 0 (VBI-0). The light responsive and auxin-autonomous cell line VBI-3 was isolated. As in the progenitor line VBI-0, cell divisions are synchronized in VBI-3 during exponential growth phase. This synchrony can be inhibited by 1-N-naphthylphthalamic acid, an auxin transport inhibitor, and this process was accompanied by the disassembly of actin filaments. However, the synchrony could be rescued when the cells were cultured under white light or with exogenous indolyl-3-acetic acid. The rescue was most efficient for continuous far-red light followed by continuous blue light, whereas continuous red light was least effective. These findings are discussed in the context of phytochrome-induced auxin biosynthesis and auxin-dependent synchrony of cell division.

Key words: Auxin transport, cell division, NPA, phytochrome, synchrony, tobacco VBI-3

Received 24 April 2009; Revised 1 October 2009 Accepted 13 October 2009


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