Journal of Experimental Botany, Vol. 53, No. 372, pp. 1237-1247,
May 15, 2002
© 2002 Oxford University Press
Original Papers |
Hydrogen peroxide and nitric oxide as signalling molecules in plants
1 Centre for Research in Plant Science, Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK
2 Centre for Research in Biomedicine, Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK
Received 10 July 2001; Accepted 21 December 2001
 |
Abstract |
|---|
 Top Abstract IntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
It is now clear that hydrogen peroxide (H2O2) and nitric oxide (NO) function as signalling molecules in plants. A wide range of abiotic and biotic stresses results in H2O2 generation, from a variety of sources. H2O2 is removed from cells via a number of antioxidant mechanisms, both enzymatic and non-enzymatic. Both biotic and abiotic stresses can induce NO synthesis, but the biosynthetic origins of NO in plants have not yet been resolved. Cellular responses to H2O2 and NO are complex, with considerable cross-talk between responses to several stimuli. In this review the potential roles of H2O2 and NO during various stresses and the signalling pathways they activate are discussed. Key signalling components that might provide targets for enhancing crop production are also identified.
Key words: Abiotic and biotic stress, hydrogen peroxide, nitric oxide, signalling molecule.
|
Introduction |
|---|
|
TopAbstractIntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
There is now compelling evidence that hydrogen peroxide (H2O2) and nitric oxide (NO) function as signalling molecules in plants (Foyer et al., 1997
; Neill et al., 1999; Bolwell, 1999; Durner and Klessig, 1999; Dat et al., 2000). H2O2 is a form of reactive oxygen species (ROS) generated as a result of oxidative stress. Oxidative stress arises from an imbalance in the generation and metabolism of ROS, with more ROS (such as H2O2) being produced than are metabolized. H2O2 is generated via superoxide, presumably in a non-controlled manner, during electron transport processes such as photosynthesis and mitochondrial respiration. H2O2 generation via electron transport is increased in response to environmental stresses such as excess excitation (light) energy, drought and cold (Bartosz, 1997; Dat et al., 2000). H2O2 and other reactive forms of oxygen derived from it can react with various cellular targets. Well established deleterious effects of ROS include damage to DNA and proteins, and lipid peroxidation (Halliwell and Gutteridge, 1989). However, plants possess a battery of antioxidant mechanisms, both enzymatic and non-enzymatic, by which ROS are removed from the cell (Noctor and Foyer, 1998). Thus, a critical balance between the production and metabolism of ROS determines the fate of the cell.
H2O2 generation is also induced in plants following exposure to a wide variety of abiotic and biotic stimuli (Fig. 1). These include extremes of temperatures, UV irradiation, excess excitation energy, ozone exposure, phytohormones such as ABA, dehydration, wounding, and elicitor and pathogen challenge (Prasad et al., 1994; Lamb and Dixon, 1997; Karpinski et al., 1999; Orozco-Cardenas and Ryan, 1999; Guan et al., 2000; Langebartels et al., 2000; Pei et al., 2000; A-H-Mackerness et al., 2001). The enzymatic sources of H2O2 generated in response to specific stimuli have not been resolved, and there is likely to be more than one. Potential sources include NADPH oxidase, cell wall peroxidases, amine oxidase, oxalate oxidase, and flavin-containing oxidases (Fig. 1; Bolwell and Wojtaszek, 1997; Bolwell et al., 2002). Whatever the source of ROS, it is now apparent that H2O2 acts as a signal to induce a range of molecular, biochemical and physiological responses within cells and plants. Some of these will be discussed in this review; it is probable that additional H2O2 responses will be characterized in the future.
|
Given that H2O2 is produced in response to such a variety of stimuli, it is likely that H2O2 mediates cross-talk between signalling pathways, and is an attractive signalling molecule contributing to the phenomenon of ‘cross-tolerance’, in which exposure of plants to one stress offers protection towards another (Bowler and Fluhr, 2000
). For example, previous exposure to sublethal doses of ozone or UV conferred tolerance to infection by a virulent pathogen (Sharma et al., 1996), and exposure to heat stress induced tolerance towards subsequent pathogen attack (Vallelian-Bindschedler et al., 1998). In addition, exposure to low levels of one stress (for example, cold) can induce tolerance towards subsequent higher levels of exposure to the same stress, a phenomenon termed acclimation tolerance (Prasad et al., 1994). Despite this cross-talk, cellular responses to various stresses do exhibit some degree of specificity as well as commonalities, and H2O2 does not induce the full range of responses induced by a ‘broader’ stress such as cold, UV or pathogen challenge (Langebartels et al., 2000; A-H-Mackerness et al., 2001; Knight and Knight, 2001). Moreover, it may be that cellular responses to H2O2 differ according to its site of synthesis or perception, for example, whether the H2O2 is synthesized in plastids or at the plasma membrane. However, the fact that oxidative stress is a common facet of many cellular stress responses, means that elucidating those intracellular signalling processes mediating H2O2 signalling is of potential significance to any programme aimed at improving crop tolerance of environmental stresses.
Nitric oxide (NO) is a free radical gas with well-characterized signalling roles in mammalian systems, acting as a second messenger during vasorelaxation, neurotransmission, immunity, and cytotoxicity (Furchgott, 1995). It is now clear that NO is also a major signal molecule in plants (Durner and Klessig, 1999), and NO signal transduction is discussed here because it seems likely that NO can be synthesized during stress responses at the same time as H2O2, and it may be that cellular effects reflect responses to both H2O2 and NO. Two landmark publications in 1998 demonstrated the role of NO during the hypersensitive response (HR) of plants to infection by bacteria and viruses (Delledonne et al., 1998; Durner et al., 1998). NO generated at the same time as H2O2 in response to pathogen attack was found to mediate defence responses similar to those seen following H2O2 generation.
|
Responses to H2O2 |
|---|
|
TopAbstractIntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
A well-established role for H2O2 is as a signal molecule during the HR (Lamb and Dixon, 1997
; Grant and Loake, 2000). H2O2 generated following pathogen challenge mediates cross-linking of cell wall proteins (Bradley et al., 1992) and plant cell wall-bound phenolics (Grant and Loake, 2000), and, although this is still somewhat controversial, may also have microbicidal function (Peng and Kuc, 1992; Wu et al., 1995). A key facet of the HR is localized programmed cell death (PCD) of host cells at the site of attempted invasion, a response that can be reproduced in suspension cultures following various treatments (McCabe and Leaver, 2000). In some systems, H2O2 has been shown to be a diffusible signal mediating localized PCD during HR (Levine et al., 1994, 1996), as well as being involved in a systemic signalling network, giving rise to ‘micro-HRs’ in systemic leaves of plants infected with avirulent bacteria (Alvarez et al., 1998). However, recent studies using transgenic catalase/ peroxidase-deficient tobacco (i.e. in which endogenous H2O2 will not be readily catabolized) showed that such plants were hyperresponsive to pathogen challenge, thus providing direct evidence for a role for H2O2 in HR cell death (Mittler et al., 1999). Recently, PCD triggered in barley aleurone by the phytohormone gibberellin was also found to be mediated by H2O2 (Bethke and Jones, 2001; Fath et al., 2002), implying a role for H2O2 in developmental PCD in addition to that induced by pathogen challenge.
In the authors' laboratory the Arabidopsis thaliana suspension culture system has been used as a model to elucidate the role of H2O2 as a signalling molecule, particularly during plant–pathogen interactions. It has been shown that H2O2 is generated following elicitor and pathogen challenge (Desikan et al., 1996; Clarke et al., 2000), and that this H2O2 acts as a signal to induce PCD and defence gene expression (Desikan et al., 1998a, 2000). Importantly, it was also found that H2O2-induced PCD requires a ‘presentation time’ of about 1 h (i.e. if H2O2 is removed within 1 h after addition, PCD can be inhibited) implying that H2O2 induces a signalling cascade leading to PCD (Desikan et al., 1998a). It is important to note that the effects of exogenous H2O2 depend on the rate at which it is degraded, which presumably determines its concentration at its site of action. Much higher concentrations of H2O2 are required to initiate PCD in Arabidopsis cells, compared to those required with protoplasts (Fig. 2A, B). The increased sensitivity of protoplasts correlates with their decreased H2O2-scavenging capacity. H2O2 is destroyed rapidly by cells: when 20 mM H2O2 is added to cells, it is degraded very quickly, with H2O2 having a half-life of 2–5 min (Fig. 2C). However, when the same dose of H2O2 is applied to protoplasts, it persists for much longer, with a half-life of about 1 h (Fig. 2D). The reduced scavenging capacity of protoplasts may be due to loss of cell wall-associated enzymes such as peroxidases.
|
PCD induced by H2O2 during the HR in Arabidopsis (Desikan et al., 1998a
) and soybean (Solomon et al., 1999) requires transcription and translation, and several studies have demonstrated that H2O2 modulates gene expression during defence responses. In soybean, H2O2 induced the expression of the defence-related genes glutathione S-transferase (GST) and glutathione peroxidase (GPX, Levine et al., 1994). In the authors’ own work, it has also been shown that, in Arabidopsis suspension cultures, H2O2 induced the expression of GST and phenylalanine ammonia-lyase (PAL, Desikan et al., 1998a). GST comprises a family of enzymes involved in cellular detoxification processes following various stresses, including oxidative stress (Marrs, 1996), glutathione peroxidases scavenge H2O2 in the ascorbate-glutathione cycle (Foyer et al., 1997), and PAL is an enzyme involved in the synthesis of defence-related compounds. Recent work also identified a tobacco gene encoding a proteasome subunit induced by H2O2 (Etienne et al., 2000); proteasomes are involved in protein degradation, a common feature of the HR cell death process.
H2O2 can induce the expression of genes potentially involved in its synthesis, such as NADPH oxidase (Desikan et al., 1998b), and also of those encoding proteins involved in its degradation, implying a complex mechanism for cellular regulation of oxidative status. H2O2 induced the expression of genes encoding ascorbate peroxidase in germinating rice embryos (Morita et al., 1999) and in Arabidopsis leaves (Karpinski et al., 1999), and wounding induced the expression of gene encoding a catalase via H2O2 in embryos and leaves of maize (Guan and Scandalios, 2000).
H2O2 is also involved in the regulation of gene expression by abiotic stresses. For example, UV-B-induced gene expression has been shown to occur via H2O2, as exposure of Arabidopsis plants to UV-B in the presence of antioxidants led to down-regulation of the UV-induced gene PDF1.2 (A-H-Mackerness et al., 1999). Systemic responses to excess excitation energy stress were found to be mediated by H2O2, indicating that it can also function as a signal during abiotic stresses (Mullineaux et al., 2000), as during pathogen-induced responses (Alvarez et al., 1998). Recent work has shown that H2O2 induces the expression of genes encoding proteins required for peroxisome biogenesis (Lopez-Huertas et al., 2000). Peroxisomes are important sources of ROS, as well as antioxidants and NO (see below), and are thus important regulators of the cellular redox state. Induction of peroxisome biogenesis genes by various stresses such as pathogen challenge and wounding (which also generates H2O2), and exogenous H2O2 (Lopez-Huertas et al., 2000) places H2O2 as a key signal molecule mediating cellular responses to stress.
In this laboratory, differential mRNA display analysis was used to identify several H2O2-induced genes in Arabidopsis suspension cultures. Up-regulated genes included those encoding a sensecence-related protein, a protein kinase and a DNA damage repair protein (Desikan et al., 2000). This work has been extended to a microarray analysis using the Arabidopsis Functional Genomics Facility (AFGC, Desikan et al., 2001a). This experiment identified a large number of up-regulated genes. As might be expected from previous work, some of these genes encode antioxidant enzymes, defence and stress-related proteins. Interestingly, genes encoding signalling proteins such as transcription factors, protein kinases and protein phosphatases were also up-regulated by H2O2; these genes were similarly induced by other stresses such as wilting, UV challenge and elicitor treatment of cells. Several genes down-regulated by H2O2 have also been identified in this work: these include genes encoding cysteine proteases, a protein kinase and photosystem-related proteins. A tobacco protein phosphatase 2C (NtPP2C1) gene is down-regulated by H2O2 and heat shock, but up-regulated by drought stress (Vranova et al., 2000). Such data highlight the complexity of signalling responses likely to be activated by H2O2.
A physiological response induced by H2O2 that has recently received much attention is stomatal closure in response to abscisic acid (ABA) and elicitors (McAinsh et al., 1996; Allan and Fluhr, 1997; Lee et al., 1999; Pei et al., 2000). Oxidative stress/H2O2 was shown to induce stomatal closure (McAinsh et al., 1996), Allan and Fluhr showed that elicitors could induce H2O2 production (via two distinct sources) (Allan and Fluhr, 1997), and Lee et al. demonstrated that both these responses were linked: elicitors caused H2O2 production which, in turn, caused stomatal closure (Lee et al., 1999). An exciting new development in this area is the recent demonstration that ABA-induced stomatal closure in Arabidopsis requires H2O2 (Pei et al., 2000). ABA induced H2O2 generation, H2O2 caused stomatal closure, and pre-treatment with diphenylene iodonium, a potential inhibitor of NADPH oxidase (Cross and Jones, 1986) and therefore H2O2 production, inhibited ABA-induced stomatal closure (Pei et al., 2000). It may be that other ABA responses are mediated, at least partly, by H2O2, for example, ABA-induced catalase gene expression in maize cells occurs via H2O2 (Guan et al., 2000). ABA-induction of guard cell H2O2 generation has also been reported for Vicia faba (Miao et al., 2000), and the authors too have found that ABA-mediated stomatal closure in pea involves H2O2 synthesis. ABA-inhibition of stomatal opening similarly seems to involve H2O2, but inhibitor work suggests that there are different H2O2 signalling pathways during closure and inhibition of opening (SJ Neill, unpublished results).
|
H2O2 signal transduction |
|---|
|
TopAbstractIntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
Calcium mobilization and reversible protein phosphorylation are ubiquitous components of eukaryotic signalling cascades. Elevations of cytosolic calcium concentrations have been shown to occur during most abiotic stresses, including oxidative stress (Knight and Knight, 2001
). Oxidative stress increased cytosolic calcium concentrations in tobacco (Price et al., 1994), and H2O2-induced calcium influx mediated stomatal closure in Commelina communis and Arabidopsis (McAinsh et al., 1996; Pei et al., 2000). Stomata of the ABA-insensitive gca mutant of Arabidopsis that do not close in response to ABA, also failed to respond to H2O2, suggesting that the mutation may be in a gene encoding a protein required for H2O2 signal transduction (Pei et al., 2000). It is likely that different ‘calcium signatures’ are invoked by different stimuli (McAinsh and Hetherington, 1998) and it may be that differing sources of H2O2 induce specific calcium responses. Previous exposure to oxidative stress altered subsequent calcium responses to drought and cold (Knight and Knight, 2001), suggesting that the intracellular calcium responses are involved in mediating cross-tolerance.
Calcium has also been implicated as an important signal following the oxidative burst in response to pathogen challenge. It was reported that H2O2 generated following pathogen challenge induces a rapid influx of calcium ions leading to apoptosis in soybean cells (Levine et al., 1996). In Arabidopsis plants infiltrated with avirulent bacteria, specific calcium ‘waves’ were found to occur concurrent with the oxidative burst, leading to the HR (Grant M et al., 2000). Calcium influx also stimulates the oxidative burst in soybean (Chandra and Low, 1997) and in tobacco cells in response to elicitor or pathogen challenge (Baker et al., 1993). The enzyme NADPH oxidase, one of the potential sources of H2O2 in plants, also has calcium binding domains (Desikan et al., 1998b; Keller et al., 1998; Torres et al., 1998). Moreover, a calcium binding protein, calmodulin, links calcium and H2O2: tobacco cells expressing a constitutively active calmodulin showed enhanced HR cell death in response to an incompatible pathogen (Harding et al., 1997). Calmodulin regulates NAD kinase activity, which generates NADPH for NADPH oxidase activity. Thus, cross-talk between H2O2 and calcium could regulate specificity and/or cross-tolerance towards various stresses (Bowler and Fluhr, 2000).
Early pharmacological data from several research groups suggested that reversible protein phosphorylation is a key event regulating the oxidative burst in response to pathogen challenge (Schwacke and Hager, 1992; Baker et al., 1993; Levine et al., 1994; Chandra and Low, 1995; Desikan et al., 1996). There are also pharmacological data to show that reversible protein phosphorylation is similarly involved in downstream signalling following H2O2 generation and/or perception (Levine et al., 1994; Rajasekhar et al., 1999; Grant JJ et al., 2000). Given the large number of protein kinases and phosphatases in plant genomes (The Arabidopsis Genome Initiative, 2000) and the complexity of signal transduction, it is likely that an interconnecting network of protein kinases and phosphatases (and other signalling components) will eventually be characterized. Moreover, it is also likely that the intracellular location of these components will be of critical importance in determining the specific outcomes of the signalling pathways that are activated by specific stimuli. As cytosolic calcium elevation is a common, early response to H2O2, it is likely that activation of calcium-dependent protein kinases and phosphatases will be an early step, with some enzymes potentially mediating downstream signalling components such as other protein kinases/phosphatases and other effector proteins. To date, though, no calcium-dependent protein kinases have been shown to be regulated by H2O2, although H2O2-regulated genes encoding protein kinases and phosphatases have been discovered (see earlier). However, it is of course possible that constitutively active calcium-dependent protein kinases are involved in H2O2 signalling.
A protein phosphorylation cascade that has been shown to be activated by H2O2 is a mitogen activated protein kinase (MAPK) cascade. MAPK cascades are evolutionarily conserved in all eukaryotes and have the typical organization shown in Fig. 3. Perception of an extracellular signal activates a MAP kinase kinase kinase (MAPKKK). This kinase then phosphorylates a MAPKK, which in turn activates a MAPK by dual phosphorylation on both threonine and tyrosine residues in a conserved T-X-Y motif (Fig. 3). Activation of the MAPK can facilitate its translocation to the nucleus where it can phosphorylate and activate transcription factors, thereby modulating gene expression (Hirt, 1997). In parsley cells, an elicitor-activated MAPK translocates to the nucleus, leading to subsequent defence responses (Ligterink et al., 1997). In plants, MAPKs can be activated in response to extracellular signals such as drought, cold, phytohormones, pathogen challenge, and osmotic stress, that lead to the activation of signal transduction pathways resulting in nuclear gene expression (Hirt, 1997). It was shown that H2O2 induces the activation of a MAPK in Arabidopsis suspension cultures (Desikan et al., 1999), and H2O2 has been shown to activate two MAPKs in Arabidopsis plants, at least one of which is activated independently of salicylic acid, jasmonate and ethylene signalling pathways (Grant JJ et al., 2000). The H2O2-activated MAPK in suspension cultures has now been identified as AtMPK6 and shown that it is activated in Arabidopsis leaves and protoplasts (Fig. 4; Desikan et al., 2001b). H2O2 also activates AtMPK6, and the related AtMPK3, in Arabidopsis leaf protoplasts (Kovtun et al., 2000). A similar activation of two MAPKs was found in suspension culture protoplasts, compared to the activation of AtMPK6 alone in cells (Fig. 4). This probably reflects the increased sensitivity of protoplasts due to their reduced H2O2-scavenging capacity (Fig. 2
).
|
|
AtMPK6 is also activated in response to elicitor challenge and cold stress (Nuhse et al., 2000
; Ichimura et al., 2000; Desikan et al., 2001b). Furthermore, ozone and H2O2 treatment induced the activation of the tobacco orthologue of AtMPK6, SIPK (Samuel et al., 2000). Using a transient gene expression system, it was demonstrated that H2O2 induced transcription driven by the specific stress-responsive promoters, GST6 and HSP18.2 (Kovtun et al., 2000). It was also shown that H2O2 activates AtMPK3/6 via ANP1, the MAPKKK at the head of the cascade. Furthermore, constitutive expression of ANP1 also activated GST6 and HSP18.2 expression. In an extension of this work, it was shown that transgenic tobacco plants over-expressing a tobacco MAPKKK orthologous to ANP1, possessed enhanced tolerance to heat shock, freezing, and salt stress, thereby demonstrating that manipulation of a key signalling component responsive to H2O2 can protect plants against various environmental stresses (Kovtun et al., 2000). Thus, various observations indicate that H2O2-activation of a MAPK cascade is a central response mediating tolerance of various stresses: firstly, that H2O2 generation occurs in response to diverse biotic and abiotic stresses; secondly, that exposure to one stress offers cross-tolerance towards another; thirdly, that there exist commonalities in defence responses to various stresses (such as MAPK activation), and, fourthly, that activation of a H2O2-regulated MAPK pathway mediates multiple stress tolerance. It is quite likely that other stress-related MAPK signalling pathways are also involved.
|
NO responses |
|---|
|
TopAbstractIntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
It has been known for some time that plants synthesize and release the gaseous molecule nitric oxide (NO) (Fig. 5
; Wildt et al., 1997) and early work suggested roles for NO in the control of root growth and as an endogenous maturation and senescence factor (Leshem et al., 1998; Ribiero et al., 1999). In animals, NO is synthesized via the enzyme nitric oxide synthase (NOS), although there may be other biochemical routes. NOS activity has been found in plants, and inhibitors of mammalian NOS shown to inhibit NO generation (Cueto et al., 1996; Ninnemann and Maier, 1996; Delledonne et al., 1998; Foissner et al., 2000; Pedroso et al., 2000). In addition, antibodies raised against mammalian NOS enzymes cross-react with plant proteins (Huang and Knopp, 1998; Barroso et al., 1999; Ribiero et al., 1999). However, no full-length NOS gene sequence has yet been isolated, and there appear to be no easily discernible NOS sequences in the Arabidopsis genome (The Arabidopsis Genome Initiative, 2000). It is possible though, that plant NOS-like enzymes do exist, with only limited sequence homology to their mammalian counterparts. An NOS enzyme activity has been purified from pea peroxisomes (Barroso et al., 1999), and a recent report that a partial cDNA clone for pea NOS has been obtained is particularly exciting (Corpas et al., 2001). Alternative sources of NO include nitrate reductase (Yamasaki and Sakihama, 2000; Wojtaszek, 2000), and there may be other, non-enzymatic routes to NO synthesis involving, for example, nitrite/ascorbate interaction or the light-mediated conversion of nitrogen dioxide to NO via carotenoids (Wojtaszek, 2000). Whatever the source of NO, it is clear that the NO-synthesis activity can be rapidly activated. Bacterial challenge induced rapid NO synthesis in soybean and Arabidopsis suspension cultures (Delledonne et al., 1998; Clarke et al., 2000) and elicitation of tobacco epidermal peels resulted in NO generation within minutes (Foissner et al., 2000).
|
So far, NO generation has been detected under conditions in which H2O2 generation is also stimulated (Delledonne et al., 1998
; Clarke et al., 2000) and it may well turn out that these two molecules are commonly present during various stresses. Thus, stress responses may reflect responses to both H2O2 and NO. In fact, bacterially-induced PCD has been reported to involve both these signals in soybean (Delledonne et al., 1998) and Arabidopsis (Clarke et al., 2000), although in the former situation the effects of NO and H2O2 were synergistic, but additive in the latter. H2O2 formation may, in some cases, occur via the superoxide radical (
). It is then possible that NO, itself a free radical (NO•) can react with to form the highly reactive peroxynitrite anion, ONOO-. Subsequent cellular effects may then be induced by peroxynitrite.
In mammals, NO has been shown to react with glutathione to form S-nitrosoglutathione (GSNO) which can serve as a systemic source of NO, and a similar situation has been suggested for plants (Fig. 5; Durner and Klessig, 1999). Interestingly, a GSNO-catabolizing enzyme (a glutathione-dependent formaldehyde dehydrogenase GS-FDH), and its encoding gene have recently been characterized (Liu et al., 2001). Mutant yeast which lacked this gene showed enhanced susceptibility to nitrosative challenge, indicating an important biological role for this enzyme (Liu et al., 2001). This gene also exists in plants—it has been cloned from pea (Shafqat et al., 1996)—so it will be of interest to determine what function, if any, this enzyme has in plants relevant to NO signalling.
A further level of complexity of H2O2 and NO signalling is clear when one remembers that they are not working alone, but in concert (or several concerts!) with other signalling molecules. Such molecules may be constitutively present, or increase in concentration/ activity (e.g. via altered cellular sensitivity) during stress, and include compounds such as ABA, jasmonic acid, ethylene, and salicylic acid (Fig. 5).
The cellular targets for NO have not been well characterized. NO can react directly with proteins via nitrosylation (Durner and Klessig, 1999). NO has been shown to inhibit the activity of tobacco aconitase, an iron–sulphur containing enzyme that regulates iron homeostasis, suggesting a role for NO in modulating iron levels in plants (Navarre et al., 2000). NO also inhibits catalase and ascorbate peroxidase activity (Clark et al., 2000). NO activates the expression of the defence-related genes PAL1, PR-1 and GST during plant–pathogen interactions (Delledonne et al., 1998; Durner et al., 1998). Another potential NO target gene is PIOX (pathogen-induced oxygenase), which is involved in redox signalling during plant defence responses (Sanz et al., 1998). This enzyme is a homologue of cyclo-oxygenases, major targets of NO in mammals (Nogawa et al., 1998). The involvement of NO in mediating UV-B induction of CHS in Arabidopsis has also been reported recently (A-H-Mackerness et al., 2001).
|
NO signal transduction |
|---|
|
TopAbstractIntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
NO signalling in mammalian cells typically involves cyclic GMP (cGMP)-dependent and independent pathways, such as protein nitrosylation (Fig. 6
). It was shown that NO signalling in tobacco required cGMP synthesis (Durner et al., 1998). NO challenge induced a transient increase in cGMP content and inhibitors of cGMP synthesis via guanylate cyclase inhibited NO-induced activation of PAL. cGMP synthesis is also required during NO-induced PCD in Arabidopsis. Inhibition of guanylate cyclase prevented NO-mediated PCD, and such inhibition could be relieved by the addition of a cell-permeable cGMP analogue, 8-bromo cGMP (Clarke et al., 2000). However, treatment with 8-bromo cGMP alone did not induce PCD, indicating that cGMP synthesis was required, but not sufficient, for the NO response. Neither guanylate cyclase nor a cGMP-dependent protein kinase (a potential target for cGMP) have yet been isolated and cloned from plants. cGMP also acts in mammalian cells via cyclic ADP ribose (cADPR). It was shown that cADPR is involved in NO-induced activation of PAL and PR-1 (Durner et al., 1998). cADPR regulated calcium levels in stomatal guard cells in response to ABA (Leckie et al., 1998), and a calcium channel blocker inhibited cADPR- and cGMP-induced responses in tobacco (Durner et al., 1998). In addition, NO activates MAP kinases in both tobacco (Kumar and Klessig, 2000) and Arabidopsis (Clarke et al., 2000). The NO-activated MAPK in tobacco can also be activated by other signals such as salicylic acid (Kumar and Klessig, 2000), and H2O2 (Samuel et al., 2000). Thus, activation of a central MAPK cascade could be a focal point of convergence of both H2O2 and NO signalling pathways activated in response to various stresses.
|
|
H2O2, NO and gene expression |
|---|
|
TopAbstractIntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
It is clear that both H2O2 and NO can mediate the transcription of specific genes. However, the exact mechanisms by which this process occurs are not yet known. It could be that H2O2 and NO have direct effects on transcription factors, for example via oxidation of cysteine residues (for H2O2) or S-nitrosylation (for NO) (Fig. 7
). Recently, a redox-sensitive transcription factor was characterized in yeast (Delaunay et al., 2000). This protein is a direct target for H2O2: oxidation by H2O2 modified its conformation and thereby activity. It is also possible that activation of transcription factors occurs via a phosphorylation cascade such as the MAPK cascade. With both H2O2 and NO activating MAPKs, this seems a likely mechanism of activating gene expression (Fig. 7).
|
|
Conclusions |
|---|
|
TopAbstractIntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
From being molecules of somewhat novelty interest, in the last few years H2O2 and NO have emerged to be central players in the world of plant cell signalling, particularly under various stressful situations. The full range of biological functions for these two signalling molecules remains to be catalogued, and determining the ways in which they interact, both together and with the ever-increasing array of signals known to be recognized by plants, will need to be elucidated. Other research priorities must include full characterization of the enzymes through which the intracellular concentrations of H2O2 and NO are regulated, and where these enzymes are located in different cells and tissues. The intracellular signalling cascades that transduce H2O2 and NO perception into cellular responses have so far been characterized only superficially. Finally, there arises the question of how H2O2 and NO are detected by cells. Such perception could conceivably involve direct interaction of H2O2 and NO with various cellular proteins, such as transcription factors, ion channels or enzymes. H2O2- and NO-sensitive enzymes could include signalling enzymes such as protein kinases and phosphatases. In mammalian cells, H2O2 modulates MAPK activity by interacting with a protein tyrosine phosphatase (Wu et al., 1998
). Given the complexity of plant cell signalling and the plethora of protein kinases and phosphatases, it will be surprising if similar examples do not exist in plants. Recent work with yeast has identified the plasma membrane-located hybrid histidine kinase SLN1 as a direct ‘peroxisensor’ that detects H2O2 (Singh, 2000). Such hybrid histidine kinases are common in plants. For example, there are eight in the Arabidopsis genome and some of these have already been identified as sensors for ethylene, cytokinin and osmotic stress (Urao et al., 2000). Thus it may well be that a hybrid histidine kinase can function as a peroxisensor in Arabidopsis—in this context it is interesting that a hybrid histidine kinase gene whose expression is increased by H2O2 has been identified (Desikan et al., 2001a). In summary, both H2O2 and NO have now been shown to function as stress signals in plants, mediating a range of responses to environmental stress. Given that such stresses impose considerable constraints on crop production, there is a real need for continued research in this area.
|
Notes |
|---|
3 To whom correspondence should be addressed. Fax: +44 (0) 117 344 2904. E-mail: Steven.Neill{at}uwe.ac.uk
|
References |
|---|
|
TopAbstractIntroductionResponses to H2O2H2O2 signal transductionNO responsesNO signal transductionH2O2, NO and gene...ConclusionsReferences |
|---|
A-H-Mackerness S, John CF, Jordan B, Thomas B. 2001. Early signalling components in ultraviolet-B responses: distinct roles for different reactive oxygen species and nitric oxide. FEBS Letters 489, 237–242.[Web of Science][Medline]
A-H-Mackerness S, Surplus SL, Blake P, John CF, Buchanan-Wollaston V, Jordan BR, Thomas B. 1999. Ultraviolet-B induced stress and changes in gene expression in Arabidopsis thaliana: role of signalling pathways controlled by jasmonic acid, ethylene and reactive oxygen species. Plant, Cell and Environment 22, 1413–1423.
Allan AC, Fluhr R. 1997. Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells. The Plant Cell 9, 1559–1572.[Abstract]
Alvarez ME, Pennell RI, Meijer P-J, Ishikawa A, Dixon RA, Lamb C. 1998. Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92, 773–784.[Web of Science][Medline]
Baker CJ, Orlandi EW, Mock NM. 1993. Harpin, an elicitor of the hypersensitive response in tobacco caused by Erwinia amylovora, elicits active oxygen production in suspension cells. Plant Physiology 102, 1341–1344.[Abstract]
Barroso JB, Corpas FJ, Carreras A, Sandalio LM, Valderrama R, Palma JM, Lupianez JA, del Rio LA. 1999. Localization of nitric oxide synthase in plant peroxisomes. Journal of Biological Chemistry 274, 36729–36733.
Bartosz G. 1997. Oxidative stress in plants. Acta Physiologia Plantarum 19, 47–64.
Bethke PC, Jones RL. 2001. Cell death of barley aleurone protoplasts is mediated by reactive oxygen species. The Plant Journal 25, 19–29.[Web of Science][Medline]
Bolwell GP. 1999. Role of active oxygen species and NO in plant defence. Current Opinion in Plant Biology 2, 287–294.[Web of Science][Medline]
Bolwell GP, Bindschedler LV, Blee KA, Butt VS, Davies DR, Gardner SL, Gerrish C, Minibayeva F. 2002. The apoplastic oxidative burst in response to biotic stress in plants: a three-component system. Journal of Experimental Botany 53, 1367–1376.
Bolwell GP, Wojtaszek P. 1997. Mechanisms for the generation of reactive oxygen species in plant defence—a broad perspective. Physiological and Molecular Plant Pathology 51, 347–366.
Bowler C, Fluhr R. 2000. The role of calcium and activated oxygens as signals for controlling cross-tolerance. Trends in Plant Science 5, 241–245.[Web of Science][Medline]
Bradley D, Kjellbom P, Lamb C. 1992. Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell 70, 21–30.[Web of Science][Medline]
Chandra S, Low PS. 1995. Role of phosphorylation in elicitation of the oxidative burst in cultured soybean cells. Proceedings of the National Academy of Sciences, USA 92, 4120–4123.
Chandra S, Low PS. 1997. Measurement of Ca2+ fluxes during elicitation of the oxidative burst in aequorin-transformed tobacco cells. Journal of Biological Chemistry 272, 28274–28280.
Clark D, Durner J, Navarre DA, Klessig DF. 2000. Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase. Molecular Plant–Microbe Interactions 13, 1380–1384.
Clarke A, Desikan R, Hurst RD, Hancock JT, Neill SJ. 2000. NO way back: nitric oxide and programmed cell death in Arabidopsis thaliana suspension cultures. The Plant Journal 24, 667–677.[Web of Science][Medline]
Corpas FJ, Barroso JB, del Rio LA. 2001. Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells. Trends in Plant Science 6, 145–150.[Web of Science][Medline]
Cross AR, Jones OTG. 1986. The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Biochemical Journal 237, 111–116.[Web of Science][Medline]
Cueto M, Hernandez-Perea O, Martin R, Benrtura ML, Rodrigo J, Lamas S, Golvano MP. 1996. Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus. FEBS Letters 398, 159–164.[Web of Science][Medline]
Dat J, Vandenbeele S, Vranova E, Van Montagu M, Inzé D, Van Breusegem F. 2000. Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences 57, 779–795.[Web of Science][Medline]
Delauney A, Isnard A-D, Toledano MB. 2000. H2O2 sensing through oxidation of the Yap1 transcription factor. EMBO Journal 19, 5157–5166.[Web of Science][Medline]
Delledonne M, Xia Y, Dixon RA, Lamb C. 1998. Nitric oxide functions as a signal in plant disease resistance. Nature 394, 585–588.[Medline]
Desikan R, A-H-Mackerness S, Hancock JT, Neill SJ. 2001a. Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiology 127, 159–172.
Desikan R, Burnett EC, Hancock JT, Neill SJ. 1998b. Harpin and hydrogen peroxide induce the expression of a homologue of gp91-phox in Arabidopsis thaliana suspension cultures. Journal of Experimental Botany 49, 1767–1771.
Desikan R, Clarke A, Hancock JT, Neill SJ. 1999. H2O2 activates a MAP kinase-like enzyme in Arabidopsis thaliana suspension cultures. Journal of Experimental Botany 50, 1863–1866.
Desikan R, Hancock JT, Coffey MJ, Neill SJ. 1996. Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase-like enzyme. FEBS Letters 382, 213–217.[Web of Science][Medline]
Desikan R, Hancock JT, Ichimura K, Shinozaki K, Neill SJ. 2001b. Harpin induces activation of the Arabidopsis MAP kinases AtMPK4 and AtMPK6. Plant Physiology 126, 1579–1587.
Desikan R, Neill SJ, Hancock JT. 2000. Hydrogen peroxide-induced gene expression in Arabidopsis thaliana. Free Radical Biology and Medicine 28, 773–778.[Web of Science][Medline]
Desikan R, Reynolds A, Hancock JT, Neill SJ. 1998a. Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on gene expression in Arabidopsis suspension cultures. Biochemical Journal 330, 115–120.
Durner J, Klessig DF. 1999. Nitric oxide as a signal in plants. Current Opinion in Plant Biology 2, 369–374.[Web of Science][Medline]
Durner J, Wendehenne D, Klessig DF. 1998. Defence gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP ribose. Proceedings of the National Academy of Sciences, USA 95, 10328–10333.
Etienne P, Petitot A-S, Houot V, Blein J-P, Suty L. 2000. Induction of tcI 7, a gene encoding a ß subunit of proteasome, in tobacco plants treated with elicitin, salicylic acid or hydrogen peroxide. FEBS Letters 466, 213–218.[Web of Science][Medline]
Fath A, Bethke P, Belligni V, Jones R. 2002. Active oxygen and cell death in cereal aleurone cells. Journal of Experimental Botany 53, 1273–1282.
Foyer CH, Lopez-Delgado H, Dat JF, Scott IM. 1997. Hydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signalling. Physiologia Plantarum 100, 241–254.
Foissner I, Wendehenne D, Langebartels C, Durner J. 2000. In vivo imaging of an elicitor-induced nitric oxide burst in tobacco. The Plant Journal 23, 817–824.[Web of Science][Medline]
Furchgott RF. 1995. Special topic: nitric oxide. Annual Review of Physiology 57, 659–682.[Web of Science][Medline]
Grant M, Brown I, Adams S, Knight M, Ainslie A, Mansfield J. 2000. The RPM1 plant disease resistance gene facilitates a rapid and sustained increase in cytosolic calcium that is necessary for the oxidative burst and hypersensitive cell death. The Plant Journal 23, 441–450.[Web of Science][Medline]
Grant JJ, Loake GJ. 2000. Role of reactive oxygen intermediates and cognate redox signaling in disease resistance. Plant Physiology 124, 21–29.
Grant JJ, Yun B-W, Loake GJ. 2000. Oxidative burst and cognate redox signalling reported by luciferase imaging: identification of a signal network that functions independently of ethylene, SA and Me-JA but is dependent on MAPKK activity. The Plant Journal 24, 569–582.[Web of Science][Medline]
Guan LM, Scandalios JG. 2000. Hydrogen peroxide-mediated catalase gene expression in response to wounding. Free Radical Biology and Medicine 28, 1182–1190.[Web of Science][Medline]
Guan LM, Zhao J, Scandalios JG. 2000. Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H2O2 is the likely intermediary signaling molecule for the response. The Plant Journal 22, 87–95.[Web of Science][Medline]
Halliwell B, Gutteridge JMC. 1989. Free radicals in biology and medicine, 2nd edn. Oxford, UK: Clarendon Press.
Harding SA, Oh S-H, Roberts DM. 1997. Transgenic tobacco expressing a foreign calmodulin gene shows an enhanced production of active oxygen species. EMBO Journal 16, 1137–1144.[Web of Science][Medline]
Hirt H. 1997. Multiple roles of MAP kinases in plant signal transduction. Trends in Plant Science 2, 11–15.[Web of Science]
Huang J-S, Knopp JA. 1998. Involvement of nitric oxide in Ralstonia solanacearum-induced hypersensitive reaction in tobacco. In: Prior P, Elphinstone J, Allen C, eds. Bacterial Wilt disease: molecular and ecological aspects. Berlin: INRA and Springer Editions, 218–224.
Ichimura K, Mizoguchi T, Yoshida R, Yuasa T, Shinozaki K. 2000. Various abiotic stresses rapidly activate Arabidopsis MAP kinases AtMPK4 and AtMPK6. The Plant Journal 24, 655–666.[Web of Science][Medline]
Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen G, Mullineaux P. 1999. Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis. Science 284, 654–657.
Keller T, Damude HG, Werner D, Doerner P, Dixon RA, Lamb C. 1998. A plant homolog of the neutrophil NADPH oxidase gp91phox subunit gene encodes a plasma membrane protein with Ca2+ binding motifs. The Plant Cell 10, 255–266.
Knight H, Knight MR. 2001. Abiotic stress signalling pathways: specificity and cross-talk. Trends in Plant Science 6, 262–267.[Web of Science][Medline]
Kovtun Y, Chiu W-L, Tena G, Sheen J. 2000. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proceedings of the National Academy of Sciences, USA 97, 2940–2945.
Kumar D, Klessig DF. 2000. Differential induction of tobacco MAP kinases by the defense signals nitric oxide, salicylic acid, ethylene, and jasmonic acid. Molecular Plant–Microbe Interactions 13, 347–351.
Lamb C, Dixon RA. 1997. The oxidative burst in plant disease resistance. Annual Review of Plant Physiology and Plant Molecular Biology 48, 251–275.[Web of Science]
Langebartels C, Schraudner M, Heller W, Ernst D, Sandermann H. 2000. Oxidative stress and defense reactions in plants exposed to air pollutants and UV-B radiation. In: Inzé D, Van Montagu M, eds. Oxidative stress in plants. Amsterdam: Harwood Academic Publishers, 105–135.
Leckie CP, McAinsh MR, Allen GJ, Sanders D, Hetherington AM. 1998. Abscisic acid-induced stomatal closure mediated by cyclic ADP-ribose. Proceedings of the National Academy of Sciences, USA 95, 15837–15842.
Lee S, Choi H, Suh S, Doo I-S, Oh K-Y, Choi EJ, Taylor ATS, Low PS, Lee Y. 1999. Oligogalacturonic acid and chitosan reduce stomatal aperture by inducing the evolution of reactive oxygen species from guard cells of tomato and Commelina communis. Plant Physiology 121, 147–152.
Leshem YY, Wills RBH, Veng-Va Ku V. 1998. Evidence for the function of the free radical gas – nitric oxide (NO) as an endogenous maturation and senescence regulating factor in higher plants. Plant Physiology and Biochemistry 36, 825–833.[Web of Science]
Levine A, Pennell RI, Alvarez ME, Palmer R, Lamb C. 1996. Calcium-mediated apoptosis in a plant hypersensitive disease resistance response. Current Biology 6, 427–437.[Web of Science][Medline]
Levine A, Tenhaken R, Dixon R, Lamb C. 1994. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79, 583–593.[Web of Science][Medline]
Ligterink W, Kroj T, zur Nieden U, Hirt H, Scheel D. 1997. Receptor-mediated activation of a MAP kinase in pathogen defense of plants. Science 275, 2054–2057.
Liu L, Hausladen A, Zeng M, Que L, Heitman J, Stamler JS. 2001. A metabolic enzyme for S-nitrosothiol conserved from bacteria to humans. Nature 410, 490–494.[Medline]
Lopez-Huertas E, Charlton WL, Johnson B, Graham IA, Baker A. 2000. Stress induces peroxisome biogenesis genes. EMBO Journal 19, 6770–6777.[Web of Science][Medline]
Marrs KA. 1996. The functions and regulation of glutathione S-transferases in plants. Annual Review of Plant Physiology and Plant Molecular Biology 47, 127–158.[Web of Science]
McAinsh MR, Clayton H, Mansfield TA, Hetherington AM. 1996. Changes in stomatal behaviour and guard cell cytosolic free calcium in response to oxidative stress. Plant Physiology 111, 1031–1042.[Abstract]
McAinsh MR, Hetherington AM. 1998. Encoding specificity in Ca2+ signalling systems. Trends in Plant Science 3, 32–36.[Web of Science]
McCabe PF, Leaver CJ. 2000. Programmed cell death in cell cultures. Plant Molecular Biology 44, 359–368.[Web of Science][Medline]
Miao Y-U, Song C-P, Dong F-C, Wang X-C. 2000. ABA-induced hydrogen peroxide generation in guard cells of Vicia faba. Acta Phytophysiologia Sinica 26, 53–58.
Mittler R, Herr EH, Orvar BL, Van Camp W, Willekens H, Inzé D, Ellis BE. 1999. Transgenic tobacco plants with reduced capability to detoxify reactive oxygen intermediates are hyperresponsive to pathogen infection. Proceedings of the National Academy of Sciences, USA 96, 14165–14170.
Morita S, Kaminaka H, Masumura T, Tanaka K. 1999. Induction of rice cytosolic ascorbate peroxidase mRNA by oxidative stress, the involvement of hydrogen peroxide in oxidative stress signalling. Plant and Cell Physiology 40, 417–422.
Mullineaux P, Ball L, Escobar C, Karpinska B, Creissen G, Karpinski S. 2000. Are diverse signalling pathways integrated in the regulation of Arabidopsis antioxidant defence gene expression in response to excess excitation energy? Philosophical Transactions of Royal Society London 355, 1531–1540.
Navarre DA, Windehenne D, Durner J, Noad R, Klessig DF. 2000. Nitric oxide modulates the activity of tobacco aconitase. Plant Physiology 122, 573–582.
Neill SJ, Desikan R, Clarke A, Hancock JT. 1999. H2O2 signalling in plant cells. In: Clavert C, Smallwood M, Bowles D, eds. Plant responses to environmental stress. UK: BIOS Scientific Publishers, 59–63.
Ninnemann H, Maier J. 1996. Indications for the occurrence of nitric oxide synthase in fungi and plants and the involvement in photoconidiation of Neurospora crassa. Photochemistry and Photobiology 64, 393–398.[Web of Science][Medline]
Noctor G, Foyer CH. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology 49, 249–279.[Web of Science]
Nogawa S, Forstner C, Zhang F, Nagayama M, Ross ME, Iadecola C. 1998. Interaction between inducible nitric oxide synthase and cyclooxygenase-2 after cerebral ischemia. Proceedings of the National Academy of Sciences, USA 95, 10966–10971.
Nuhse TS, Peck SC, Hirt H, Boller T. 2000. Microbial elicitors induce activation and dual phosphorylation of the Arabidopsis thaliana MAPK6. Journal of Biological Chemistry 275, 7521–7526.
Orozco-Cardenas M, Ryan C. 1999. Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. Proceedings of the National Academy of Sciences, USA 96, 6553–6557.
Pedroso MC, Magalhaes JR, Durzan D. 2000. A nitric oxide burst precedes apoptosis in angiosperm and gymnosperm callus cells and foliar tissues. Journal of Experimental Botany 51, 1027–1036.
Pei Z-M, Murata Y, Benning G, Thomine S, Klusener B, Allen GJ, Grill E, Schroeder JI. 2000. Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406, 731–734.[Medline]
Peng M, Kuc J. 1992. Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks. Phytopathology 82, 2494–2498.
Prasad TK, Anderson MD, Martin BA, Stewart CR. 1994. Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. The Plant Cell 6, 65–74.[Abstract]
Price AH, Taylor A, Ripley SJ, Griffiths A, Trewavas AJ, Knight MR. 1994. Oxidative signals in tobacco increase cytosolic calcium. The Plant Cell 6, 1301–1310.[Abstract]
Rajasekhar VK, Lamb C, Dixon RA. 1999. Early events in the signal pathway for the oxidative burst in soybean cells exposed to avirulent Pseudomonas syringae pv glycinea. Plant Physiology 120, 1137–1146.
Ribiero EA, Cunha FQ, Tamashiro WMSC, Martin IS. 1999. Growth phase-dependent subcellular localization of nitric oxide synthase in maize cells. FEBS Letters 445, 283–286.[Web of Science][Medline]
Samuel MA, Miles GP, Ellis BE. 2000. Ozone treatment rapidly activates MAP kinase signalling in plants. The Plant Journal 22, 367–376.[Web of Science][Medline]
Sanz A, Moreno JI, Castresana C. 1998. PIOX, a new pathogen-induced oxygenase with homology to animal cyclooxygenase. The Plant Cell 10, 1523–1537.
Schwacke R, Hager A. 1992. Fungal elicitors induce a transient release of active oxygen species from cultured spruce cells that is dependent on Ca2+ and protein kinase activity. Planta 187, 136–141.[Web of Science]
Shafqat J, El-Ahmad M, Danielsson O, Martinez MC, Persson B, Pares X, Jornvall H. 1996. Pea formaldehyde-active class III alcohol dehydrogenase: common derivation of the plant and animal forms but not of the corresponding ethanol-active forms (classes I and P). Proceedings of the National Academy of Sciences, USA 93, 5595–5599.
Sharma YK, Leon J, Raskin I, Davis KR. 1996. Ozone-induced responses in Arabidopsis thaliana: the role of salicylic acid in the accumulation of defense-related transcripts and induced resistance. Proceedings of the National Academy of Sciences, USA 93, 5099–5104.
Singh KK. 2000. The Saccharomyces cerevisiae SLN1P-SSK1P two-component system mediates response to oxidative stress and in an oxidant-specific fashion. Free Radical Biology and Medicine 29, 1043–1050.[Web of Science][Medline]
Solomon M, Belenghi B, Delledonne M, Menachem E, Levine A. 1999. The involvement of cysteine proteases and protease inhibitor genes in the regulation of programmed cell death in plants. The Plant Cell 11, 431–443.
The Arabidopsis Genome Initiative. 2000. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796–815.[Medline]
Torres MA, Onuchi H, Hamada S, Machida C, Hammond-Kosack KE, Jones JDG. 1998. Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox). The Plant Journal 14, 365–370.[Web of Science][Medline]
Urao T, Yamaguchi-Shinozaki K, Shinozaki K. 2000. Two-component systems in plant signal transduction. Trends in Plant Science 5, 67–74.[Web of Science][Medline]
Vallelian-Bindschedler L, Schweizer P, Mosinger E, Metraux J-P. 1998. Heat-induced resistance to powdery mildew (Blumeria graminis f.sp. hordei) is associated with a burst of active oxygen species. Physiological and Molecular Plant Pathology 52, 185–199.[Web of Science]
Vranova E, Langebartels C, Van Montagu M, Inzé D, Van Camp W. 2000. Oxidative stress, heat shock and drought differentially affect expression of a tobacco protein phosphatase 2C. Journal of Experimental Botany 51, 1763–1764.
Wildt J, Kley D, Rockel A, Rockel P, Segschneider HJ. 1997. Emission of NO from several higher plant species. Journal of Geophysical Research 102, 5919–5927.
Wojtaszek P. 2000. Nitric oxide in plants: to NO or not to NO. Phytochemistry 54, 1–4.[Web of Science][Medline]
Wu Y, Kwon K-S, Rhee SG. 1998. Probing cellular targets of H2O2 with fluorescein-conjugated iodoacetamide and antibodies to fluorescein. FEBS Letters 440, 111–115.[Web of Science][Medline]
Wu G, Shortt BJ, Lawrence EB, Fitzsimmons KC, Shah DM. 1995. Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in transgenic potato plants. The Plant Cell 7, 1357–1368.[Abstract]
Yamasaki H, Sakihama Y. 2000. Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase: in vitro evidence for the NR-dependent formation of active nitrogen species. FEBS Letters 468, 89–92.[Web of Science][Medline]
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Zheng, K. Miao, Y. Zhang, S. Pan, M. Zhang, and H. Jiang Nitric oxide mediates the fungal-elicitor-enhanced biosynthesis of antioxidant polyphenols in submerged cultures of Inonotus obliquus Microbiology, October 1, 2009; 155(10): 3440 - 3448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Wong and K. Shimamoto Sending ROS on a Bullet Train Sci. Signal., September 29, 2009; 2(90): pe60 - pe60. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. F. Walton, R.-M. Wu, A. C. Richardson, M. Davy, R. P. Hellens, K. Thodey, B. J. Janssen, A. P. Gleave, G. M. Rae, M. Wood, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds J. Exp. Bot., September 1, 2009; 60(13): 3835 - 3848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhang, Q. Fang, Z. Zhang, Y. Wang, and X. Zheng The role of respiratory burst oxidase homologues in elicitor-induced stomatal closure and hypersensitive response in Nicotiana benthamiana J. Exp. Bot., July 1, 2009; 60(11): 3109 - 3122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lee, J. C. Chiecko, S. A. Kim, E. L. Walker, Y. Lee, M. L. Guerinot, and G. An Disruption of OsYSL15 Leads to Iron Inefficiency in Rice Plants Plant Physiology, June 1, 2009; 150(2): 786 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Muscolo and M. Sidari Carboxyl and Phenolic Humic Fractions Affect Pinus Nigra Callus Growth and Metabolism Soil Sci. Soc. Am. J., May 13, 2009; 73(4): 1119 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Almagro, L. V. Gomez Ros, S. Belchi-Navarro, R. Bru, A. Ros Barcelo, and M. A. Pedreno Class III peroxidases in plant defence reactions J. Exp. Bot., February 1, 2009; 60(2): 377 - 390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Steffens and M. Sauter Epidermal Cell Death in Rice Is Confined to Cells with a Distinct Molecular Identity and Is Mediated by Ethylene and H2O2 through an Autoamplified Signal Pathway PLANT CELL, January 1, 2009; 21(1): 184 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Feng, X. Li, J. Duan, H. Li, and H. Liang Chilling Tolerance of Wheat Seedlings Is Related to an Enhanced Alternative Respiratory Pathway Crop Sci., November 24, 2008; 48(6): 2381 - 2388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hirayama, S. Cho, and P. Sassone-Corsi Circadian control by the reduction/oxidation pathway: Catalase represses light-dependent clock gene expression in the zebrafish PNAS, October 2, 2007; 104(40): 15747 - 15752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xing, W. Jia, and J. Zhang AtMEK1 mediates stress-induced gene expression of CAT1 catalase by triggering H2O2 production in Arabidopsis J. Exp. Bot., August 28, 2007; (2007) erm144v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Laxalt, N. Raho, A. t. Have, and L. Lamattina Nitric Oxide Is Critical for Inducing Phosphatidic Acid Accumulation in Xylanase-elicited Tomato Cells J. Biol. Chem., July 20, 2007; 282(29): 21160 - 21168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rodriguez-Serrano, M. C. Romero-Puertas, G. M. Pastori, F. J. Corpas, L. M. Sandalio, L. A. del Rio, and J. M. Palma Peroxisomal membrane manganese superoxide dismutase: characterization of the isozyme from watermelon (Citrullus lanatus Schrad.) cotyledons J. Exp. Bot., July 1, 2007; 58(10): 2417 - 2427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, A. Takemiya, T. Kinoshita, and K.-i. Shimazaki Nitric Oxide Inhibits Blue Light-Specific Stomatal Opening Via Abscisic Acid Signaling Pathways in Vicia Guard Cells Plant Cell Physiol., May 1, 2007; 48(5): 715 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mazzitelli, R. D. Hancock, S. Haupt, P. G. Walker, S. D. A. Pont, J. McNicol, L. Cardle, J. Morris, R. Viola, R. Brennan, et al. Co-ordinated gene expression during phases of dormancy release in raspberry (Rubus idaeus L.) buds J. Exp. Bot., March 1, 2007; 58(5): 1035 - 1045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ali, W. Ma, F. Lemtiri-Chlieh, D. Tsaltas, Q. Leng, S. von Bodman, and G. A. Berkowitz Death Don't Have No Mercy and Neither Does Calcium: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and Innate Immunity PLANT CELL, March 1, 2007; 19(3): 1081 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yang, Y. Cheng, R. Ji, and C. Zhang Novel model of inflammatory neointima formation reveals a potential role of myeloperoxidase in neointimal hyperplasia Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3087 - H3093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jasid, M. Simontacchi, C. G. Bartoli, and S. Puntarulo Chloroplasts as a Nitric Oxide Cellular Source. Effect of Reactive Nitrogen Species on Chloroplastic Lipids and Proteins Plant Physiology, November 1, 2006; 142(3): 1246 - 1255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. d. l. M. Dana, J. A. Pintor-Toro, and B. Cubero Transgenic Tobacco Plants Overexpressing Chitinases of Fungal Origin Show Enhanced Resistance to Biotic and Abiotic Stress Agents Plant Physiology, October 1, 2006; 142(2): 722 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Cheeseman Hydrogen peroxide concentrations in leaves under natural conditions J. Exp. Bot., July 1, 2006; 57(10): 2435 - 2444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-J. Park, Z. Jeknic, and T. H. H. Chen Exogenous Application of Glycinebetaine Increases Chilling Tolerance in Tomato Plants Plant Cell Physiol., June 1, 2006; 47(6): 706 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Zaninotto, S. L. Camera, A. Polverari, and M. Delledonne Cross Talk between Reactive Nitrogen and Oxygen Species during the Hypersensitive Disease Resistance Response Plant Physiology, June 1, 2006; 141(2): 379 - 383. [Full Text] [PDF]
|
|
|
|
|
|
A. Zhang, M. Jiang, J. Zhang, M. Tan, and X. Hu Mitogen-Activated Protein Kinase Is Involved in Abscisic Acid-Induced Antioxidant Defense and Acts Downstream of Reactive Oxygen Species Production in Leaves of Maize Plants Plant Physiology, June 1, 2006; 141(2): 475 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Zago, S. Morsa, J. F. Dat, P. Alard, A. Ferrarini, D. Inze, M. Delledonne, and F. Van Breusegem Nitric Oxide- and Hydrogen Peroxide-Responsive Gene Regulation during Cell Death Induction in Tobacco Plant Physiology, June 1, 2006; 141(2): 404 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-J. Dietz, S. Jacob, M.-L. Oelze, M. Laxa, V. Tognetti, S. M. N. de Miranda, M. Baier, and I. Finkemeier The function of peroxiredoxins in plant organelle redox metabolism J. Exp. Bot., May 1, 2006; 57(8): 1697 - 1709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Pauly, C. Pucciariello, K. Mandon, G. Innocenti, A. Jamet, E. Baudouin, D. Herouart, P. Frendo, and A. Puppo Reactive oxygen and nitrogen species and glutathione: key players in the legume-Rhizobium symbiosis J. Exp. Bot., May 1, 2006; 57(8): 1769 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Marino, E. M. Gonzalez, and C. Arrese-Igor Drought effects on carbon and nitrogen metabolism of pea nodules can be mimicked by paraquat: evidence for the occurrence of two regulation pathways under oxidative stresses J. Exp. Bot., February 1, 2006; 57(3): 665 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Clarke, L. A. J. Mur, R. M. Darby, and P. Kenton Harpin modulates the accumulation of salicylic acid by Arabidopsis cells via apoplastic alkalization J. Exp. Bot., December 1, 2005; 56(422): 3129 - 3136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhou, Z. Guo, J. Xing, and B. Huang Nitric oxide is involved in abscisic acid-induced antioxidant activities in Stylosanthes guianensis J. Exp. Bot., December 1, 2005; 56(422): 3223 - 3228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-Q. Guo and N. M. Crawford Arabidopsis Nitric Oxide Synthase1 Is Targeted to Mitochondria and Protects against Oxidative Damage and Dark-Induced Senescence PLANT CELL, December 1, 2005; 17(12): 3436 - 3450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-T. Shiu and T.-M. Lee Ultraviolet-B-induced oxidative stress and responses of the ascorbate-glutathione cycle in a marine macroalga Ulva fasciata J. Exp. Bot., November 1, 2005; 56(421): 2851 - 2865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Wolucka, A. Goossens, and D. Inze Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions J. Exp. Bot., September 1, 2005; 56(419): 2527 - 2538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pasternak, G. Potters, R. Caubergs, and M. A. K. Jansen Complementary interactions between oxidative stress and auxins control plant growth responses at plant, organ, and cellular level J. Exp. Bot., August 1, 2005; 56(418): 1991 - 2001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-X. Yang, R.-Y. Wang, F. Ren, J. Liu, J. Cheng, and Y.-T. Lu AtGLB1 Enhances the Tolerance of Arabidopsis to Hydrogen Peroxide Stress Plant Cell Physiol., August 1, 2005; 46(8): 1309 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Shin, R. H. Berg, and D. P. Schachtman Reactive Oxygen Species and Root Hairs in Arabidopsis Root Response to Nitrogen, Phosphorus and Potassium Deficiency Plant Cell Physiol., August 1, 2005; 46(8): 1350 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Montillet, S. Chamnongpol, C. Rusterucci, J. Dat, B. van de Cotte, J.-P. Agnel, C. Battesti, D. Inze, F. Van Breusegem, and C. Triantaphylides Fatty Acid Hydroperoxides and H2O2 in the Execution of Hypersensitive Cell Death in Tobacco Leaves Plant Physiology, July 1, 2005; 138(3): 1516 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lanfranco, M. Novero, and P. Bonfante The Mycorrhizal Fungus Gigaspora margarita Possesses a CuZn Superoxide Dismutase That Is Up-Regulated during Symbiosis with Legume Hosts Plant Physiology, April 1, 2005; 137(4): 1319 - 1330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Casolo, E. Petrussa, J. Krajnakova, F. Macri, and A. Vianello Involvement of the mitochondrial KATP+ channel in H2O2- or NO-induced programmed death of soybean suspension cell cultures J. Exp. Bot., March 1, 2005; 56(413): 997 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lindermayr, G. Saalbach, and J. Durner Proteomic Identification of S-Nitrosylated Proteins in Arabidopsis Plant Physiology, March 1, 2005; 137(3): 921 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Chaves and M. M. Oliveira Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture J. Exp. Bot., November 1, 2004; 55(407): 2365 - 2384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Corpas, J. B. Barroso, A. Carreras, M. Quiros, A. M. Leon, M. C. Romero-Puertas, F. J. Esteban, R. Valderrama, J. M. Palma, L. M. Sandalio, et al. Cellular and Subcellular Localization of Endogenous Nitric Oxide in Young and Senescent Pea Plants Plant Physiology, September 1, 2004; 136(1): 2722 - 2733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Barth, W. Moeder, D. F. Klessig, and P. L. Conklin The Timing of Senescence and Response to Pathogens Is Altered in the Ascorbate-Deficient Arabidopsis Mutant vitamin c-1 Plant Physiology, April 1, 2004; 134(4): 1784 - 1792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mahadevan, J. Lappe, R. T. Rhyne, N. D. Cruz-Bermudez, E. Marder, and M. F. Goy Nitric Oxide Inhibits the Rate and Strength of Cardiac Contractions in the Lobster Homarus americanus by Acting on the Cardiac Ganglion J. Neurosci., March 17, 2004; 24(11): 2813 - 2824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kuzniak and M. Sklodowska The effect of Botrytis cinerea infection on the antioxidant profile of mitochondria from tomato leaves J. Exp. Bot., March 1, 2004; 55(397): 605 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sagi, O. Davydov, S. Orazova, Z. Yesbergenova, R. Ophir, J. W. Stratmann, and R. Fluhr Plant Respiratory Burst Oxidase Homologs Impinge on Wound Responsiveness and Development in Lycopersicon esculentum PLANT CELL, March 1, 2004; 16(3): 616 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yoshida and K. Hasunuma Reactive Oxygen Species Affect Photomorphogenesis in Neurospora crassa J. Biol. Chem., February 20, 2004; 279(8): 6986 - 6993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cabane, J.-C. Pireaux, E. Leger, E. Weber, P. Dizengremel, B. Pollet, and C. Lapierre Condensed Lignins Are Synthesized in Poplar Leaves Exposed to Ozone Plant Physiology, February 1, 2004; 134(2): 586 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Desikan, M.-K. Cheung, J. Bright, D. Henson, J. T. Hancock, and S. J. Neill ABA, hydrogen peroxide and nitric oxide signalling in stomatal guard cells J. Exp. Bot., January 2, 2004; 55(395): 205 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Ratnayaka, W. T. Molin, and T. M. Sterling Physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought J. Exp. Bot., October 1, 2003; 54(391): 2293 - 2305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Navabpour, K. Morris, R. Allen, E. Harrison, S. A-H-Mackerness, and V. Buchanan-Wollaston Expression of senescence-enhanced genes in response to oxidative stress J. Exp. Bot., October 1, 2003; 54(391): 2285 - 2292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Matamoros, D. A. Dalton, J. Ramos, M. R. Clemente, M. C. Rubio, and M. Becana Biochemistry and Molecular Biology of Antioxidants in the Rhizobia-Legume Symbiosis Plant Physiology, October 1, 2003; 133(2): 499 - 509. [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang, C. Wang, C. Qin, T. Wood, G. Olafsdottir, R. Welti, and X. Wang The Oleate-Stimulated Phospholipase D, PLD{delta}, and Phosphatidic Acid Decrease H2O2-Induced Cell Death in Arabidopsis PLANT CELL, October 1, 2003; 15(10): 2285 - 2295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gilliland, D. P. Singh, J. M. Hayward, C. A. Moore, A. M. Murphy, C. J. York, J. Slator, and J. P. Carr Genetic Modification of Alternative Respiration Has Differential Effects on Antimycin A-Induced versus Salicylic Acid-Induced Resistance to Tobacco mosaic virus Plant Physiology, July 1, 2003; 132(3): 1518 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Desikan, R. Griffiths, J. Hancock, and S. Neill A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsisthaliana PNAS, December 10, 2002; 99(25): 16314 - 16318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schutzendubel and A. Polle Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization J. Exp. Bot., May 15, 2002; 53(372): 1351 - 1365. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||