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3D Deconvolution Image Data from five different nuclei. Two are from desyanptic1 (dsy1) pollen mother cells, and three are from desynaptic (dy). =========================================================================================== File Name Mutant Stage HBID Fig. Description ------------------------------------------------------------------------------------------- dsy1_ZY_DFO.JPG [JPG] dsy1_ZY_D [AVI][MOV] dsy1 Zygotene 5s3b 1A grey-scale sections, DAPI (DNA) dsy1_ZY_F [AVI][MOV] " " " " grey-scale sections, FITC (telomeres) dsy1_ZY_O [AVI][MOV] " " " " grey-scale sections, Rhod. (5S rDNA) dsy1_ZY_DFO [AVI][MOV] " " " " color sections, DAPI/FITC/RHOD(5S rDNA) = Blue/Green/Red dsy1_ZY_360 [AVI][MOV] " " " " color, spinning projections DAPI/FITC/RHOD(5S rDNA) = Blue/Green/Red dsy1_DP_DO.JPG [JPG] dsy1_DP_D [AVI][MOV] dsy1 Diplotene 5s3d - grey-scale sections, DAPI (DNA) dsy1_DP_F [AVI][MOV] " " " - grey-scale sections, FITC (telomeres) dsy1_DP_O [AVI][MOV] " " " - grey-scale sections, Rhod. (5S rDNA) dsy1_DP_DFO [AVI][MOV] " " " - color sections, DAPI/FITC/RHOD = Blue/Green/Red dsy1_DP_360 [AVI][MOV] " " " - color, spinning projections DAPI/FITC/RHOD = Blue/Green/Red dy_LE_DFO.JPG [JPG] dy_LE_D [AVI][MOV] dy Leptotene 1t3e - grey-scale sections, DAPI (DNA) dy_LE_F [AVI][MOV] " " " - grey-scale sections, FITC (telomeres) dy_LE_O [AVI][MOV] " " " - grey-scale sections, Rhod. (5S rDNA) dy_LE_DFO [AVI][MOV] " " " - color sections, DAPI/FITC/RHOD = Blue/Green/Red dy_ZY_DFO.JPG [JPG] dy_ZY_D [AVI][MOV] dy Zygotene 1t3d 2A grey-scale sections, DAPI (DNA) dy_ZY_F [AVI][MOV] " " " " grey-scale sections, FITC (telomeres) dy_ZY_O [AVI][MOV] " " " " grey-scale sections, Rhod. (5S rDNA) dy_ZY_DFO [AVI][MOV] " " " " color sections, DAPI/FITC/RHOD Blue/Green/Red dy_PA_DFO.JPG [JPG] dy_PA_D [AVI][MOV] dy Pachytene 2t3e 2B grey-scale sections, DAPI (DNA) dy_PA_F [AVI][MOV] " " " " grey-scale sections, FITC (telomeres) dy_PA_O [AVI][MOV] " " " " grey-scale sections, Rhod. (5s rDNA) dy_PA_DFO [AVI][MOV] " " " " color sections, DAPI/FITC/RHOD = Blue/Green/Red dy_PA_360 [AVI][MOV] " " " " color, spinning projections, DAPI/FITC red/green =========================================================================================== Table notes: Single (JPG) images are greyscale projections of whole cell or nucleus subset images (DAPI, FITC, and Rhod.) 'grey-scale sections' show the entire 3D subset of data (optical sections) for a particular wavelength (D for DAPI, F for FITC, and O for Rhodamine). 'color sections' show the entire 3D subset with psuedocolor overlay of all three wavelengths 'color, spinning projections' show through focus projections viewed from a series of angles about the Y axis, these appear to spin when played through in sequence. [AVI] are AVI movie format, [MOV] QuickTime movie format =================================================================================
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