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Table 1. Primer sequences of seven housekeeping genes and one gene of interest, the amplification length and the melting temperature of the amplified product

Name
Accession number
Primer sequence 5'-3'
Primer sequence 5'-3'
Length (bp)
Tm (°C)
ß-tubulin 609267 ATGTTCAGGCGCAAGGCTT TCTGCAACCGGGTCATTCAT 101 79
ef1{alpha} AB061263 ATTGGAAACGGATATGCTCCA TCCTTACCTGAACGCCTGTCA 101 79
L2 39816659 GGCGAAATGGGTCGTGTTAT CATTTCTCTCGCCGAAATCG 121 82
18S rRNA X67238 GGGCATTCGTATTTCATAGTCAGAG CGGTTCTTGATTAATGAAAACATCCT 101 75
aprt CK270447 GAACCGGAGCAGGTGAAGAA GAAGCAATCCCAGCGATACG 121 80
Actin X55749 GCTTCCCGATGGTCAAGTCA GGATTCCAGCTGCTTCCATTC 101 81
Cyclophilin AF126551 CTCTTCGCCGATACCACTCC TCACACGGTGGAAGGTTGAG 121 81
Hsp20.2
 BQ511516
 TGTTGAAGTTGGGTCTTAGCATAGAAG
 CCTCCAGTGCAGGCATGTC
 76
 78





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