© 1960 Oxford University Press
RESEARCH-ARTICLE |
The Nature of the Resistance of Oats to the Take-all Fungus
III. DISTRIBUTION OF THE INHIBITOR IN OAT SEEDLINGS
St. Anne's College Oxford
The distribution in oat seedlings of an inhibitor of fungal growth and respiration is described. The inhibitors from roots and leaves differ in that that from roots has a bright blue fluorescence under ultra-violet light, while in other respects they behave similarly; it is suggested that they differ in structure of a part of the molecule not concerned in the biological activity. The inhibitor is present in high concentration in the meristems of roots and leaves, one root tip extracted in 2 ml. water being sufficient to reduce growth of O. graminis to 50 per cent. of the control; yield from other parts of the seedling is much less. A quick assay technique based on growth-rate of mycelium is described, also a method for direct identification of inhibitory zones on chromatograms which avoids the necessity for elution.
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