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© 1979 Oxford University Press

RESEARCH-ARTICLE

An Improved Method for Estimating Macromolecular Synthesis1,2

DUSHYANT K. GULATI, GERALD A. ROSENTHAL and P. S. SABHARWAL

T. H. Morgan School of Biological Sciences, University of Kentucky Lexington, Kentucky 40506

Estimating rates of DNA, RNA, and protein synthesis has been greatly facilitated by the use of radioactive precursors. Currently available methods to assess macromolecular synthesis in higher plants are time-consuming and imprecise. In the procedure outlined here, the conventional method is simplified by processing the tissue intact. This method allows a rapid analysis and considerably reduces the inherent variability associated with the conventional method.


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