Journal of Experimental Botany, Vol 49, 573-582, Copyright © 1998 by Oxford University Press
X Xu, D Vreugdenhil and A van Lammeren
Cell division and cell enlargement were studied to reveal the developmental
mechanism of potato tuberization using both in vivo
in vitro culture systems. Distribution of cells in
S-phase was visualized by immunolabelling of incorporated bromodeoxyuridine
(BrdU). Mitosis was detected in DAPI (4,6-di-amidino-2-phenylindole) or
toluidine blue-stained sections. Timing and frequency of cell division were
determined by daily cell counting, and cell enlargement was deduced from
measurements of cell diameters.Under in vivo
conditions, lateral underground buds developed into stolons due to
transverse cell divisions and cell elongation in the apical region of the
buds. At the onset of tuber formation, the elongation of stolons stopped
and cells in pith and cortex enlarged and divided longitudinally, resulting
in the swelling of the stolon tip. When tubers had a diameter of 0.8 cm,
longitudinal divisions had stopped but randomly oriented division and cell
enlargement occurred in the perimedullary region and continued until tubers
reached their final diameter.In vitro tubers were
formed by axillary buds on single node cuttings cultured under
tuber-including conditions. They stopped growing at a diameter of 0.8 cm.
Pith and cortex were involved in tuberization such as that found during the
early stage of in vivo tuberization (<0.8 cm in
diameter). The larger size of in vivo tubers is,
however, due to further development of the perimedullary region, which is
lacking in vitro conditions.Keywords:
Cell division, cell enlargement, DNA synthesis, in
vitro culture, potato, tuber formation.
ARTICLES
Cell division and cell enlargement during potato tuber formation
Department of Plant Physiology, Department of Plant Cytology and Morphology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, PR China; Corresponding author; Fax: 31 317 484318; E-mail: andre.vanlammeren@algem.pcm.wau.nl
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