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Journal of Experimental Botany, Vol 49, 1129-1137, Copyright © 1998 by Oxford University Press

ARTICLES

Light-induced changes of cytosolic pH in Eremosphaera viridis: recordings and kinetic analysis

B Bethmann, W Simonis and G Schonknecht
Molecular Plant Physiology and Biophysics, Botany I, University of Wurzburg, Julius-von-Sachs Platz 2, D-97082 Wurzburg, Germany; Corresponding author; e-mail: gerald@botanik.uni-wuerzburg.de

Short-term light-induced pH changes were measured by means of pH-selective microelectrodes in the uni-cellular green alga Eremosphaera viridis. Cytosolic pH changes were always transient and reversible within 1-3 min. Taking into account the low-pass filtering by the experimental set-up, the light-dependent cytosolic pH changes could be described by a sum of two exponential functions. By mathematical analysis it is demonstrated that the transient nature of light-dependent pH changes was due to a consecutive pH-stat regulation and not to a parallel light-triggered reaction. The short-term pH regulation depended linearly on the pH-deviation with no indication of a feedback control loop. Vacuolar light-dependent pH changes were measured to investigate further which pH-stat mechanisms are responsible for the back regulation of the light-dependent cytosolic pH changes. Vacuolar pH changes were too small to explain the recovery of the cytosolic pH after darkening or illumination by H+ fluxes across the tonoplast.Keywords: Eremosphaera viridis, ion-selective microelectrodes, light-induced pH-changes, pH-regulation, vacuolar pH.
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