Journal of Experimental Botany, Vol 49, 1139-1146, Copyright © 1998 by Oxford University Press
M Altamura, S D'Angeli and F Capitani
The objective was to determine whether the protein of
rolB affects shoot formation and whether this
potential relationship depends on the developmental stages of the plant
and/or on the culture conditions. Thin cell layers (TCL) and leaf explants
were excised from tobacco plants in the vegetative and flowering stages and
cultured under various hormonal conditions. In TCLs of vegetative-stage
plants, the expression of rolB enhanced the formation
of the shoot buds under hormone-free conditions and with specific
concentrations of auxin and/or cytokinin. Histological examination showed
that the induction of the shoot meristemoids was particularly enhanced by
rolB protein and that meristemoid growth was
accelerated. In leaf explants from vegetative-stage plants, the expression
of rolB increased the formation of shoot buds in the
presence of 1
ARTICLES
The protein of rolB gene enhances shoot formation in tobacco leaf explants and thin cell layers from plants in different physiological states
Dipartimento di Biologia Vegetale, Universita 'La Sapienza', P. le A Moro 5, 00185 Rome, Italy; Corresponding author; e-mail: altamuram@axrma.uniroma.it
M IAA plus 1 or 10 M cytokinin. With BA alone, at a 0.1 M concentration, shoot formation occurred in the
transgenic explants only, whereas with concentrations ranging from 0.5 to
10 M, it was higher in these explants than in
controls.RolB protein enhanced the formation of shoot
buds in TCLs from flowering plants under all hormonal conditions. In the
presence of 1 
M IAA and kinetin, the protein also increased the
flowering response. In leaf explants from flowering plants, the expression
of rolB increased the number of shoot buds in the
presence of 1 M IAA with 10 M BA.In conclusion,
rolB protein promotes shoot formation; it seems to
have a positive interaction with cytokinin and an effect on the induction
of the meristematic condition.Keywords: Shoot
formation, in vitro culture, leaf explants,
rolB protein, tobacco thin cell layers.
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