Journal of Experimental Botany, Vol 49, 1191-1202, Copyright © 1998 by Oxford University Press
F Booker and J Miller
Plants treated with the air pollutant, ozone (O3), often respond with
increased transcript levels and activities of enzymes in the general
phenylpropanoid and lignin pathways. This suggests that increased
biosynthesis of lignin and related products also occurs. The purpose of
this study was to determine whether O3 stimulated enzyme activities in
these pathways in soybean [Glycine max (L.) Merr.]
leaves, and if so, were hydroxycinnamic acids, lignin and suberin also
produced. Plants were grown for 6 weeks in charcoal-filtered (CF) air and
then treated with either CF air or CF air plus 100 nmol O3
mol-1 7 h daily for up to 13 d in chambers in the
greenhouse or in open-top chambers in the field. In greenhouse experiments,
the activities of general phenylpropanoid pathway enzymes (phenylalanine
ammonia-lyase and 4-coumarate:COA ligase) were stimulated by O3 after 6 h.
The activity of an enzyme in the lignin pathway (cinnamyl alcohol
dehydrogenase) increased in O3-treated plants after 27 h. In greenhouse and
field experiments, levels of cell-wall-bound total phenolics,
acid-insoluble lignin and lignothioglycolic acid (LTGA) extracted from leaf
tissue from O3-treated plants increased on average by 65%. However,
histochemistry, UV and IR spectra, radiolabelling and a nitrobenzene
oxidation assay all indicated that lignin and suberin did not increase with
O3 treatment. Acid-insoluble lignin and LTGA extracted from O3-treated
plants probably contained phenolic polymers that form in wounded or
senescent tissues, thereby causing overestimates of the changes.
Ozone-induced increases in phenolic metabolism, resembling certain elicited
defence responses, thus occurred in concert with effects characteristic of
the browning reaction and wound responses.Keywords:
Phenolics, lignin, ozone, soybean, air pollution.
ARTICLES
Phenylpropanoid metabolism and phenolic composition of soybean [Glycine max (L.) Merr.] leaves following exposure to ozone
US Department of Agriculture, Agricultural Research Service, Air Quality Research Unit, and Department of Crop Science, Box 7632, North Carolina State University, Raleigh, NC 27695, USA; Corresponding author; e-mail: fitz@unity.ncsu.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  F. L. Booker, K. O. Burkey, W. A. Pursley, and A. S. Heagle Elevated Carbon Dioxide and Ozone Effects on Peanut: I. Gas-Exchange, Biomass, and Leaf Chemistry Crop Sci., July 30, 2007; 47(4): 1475 - 1487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Veljovic-Jovanovic, B. Kukavica, B. Stevanovic, and F. Navari-Izzo Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica J. Exp. Bot., May 1, 2006; 57(8): 1759 - 1768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cabane, J.-C. Pireaux, E. Leger, E. Weber, P. Dizengremel, B. Pollet, and C. Lapierre Condensed Lignins Are Synthesized in Poplar Leaves Exposed to Ozone Plant Physiology, February 1, 2004; 134(2): 586 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Babu, T. A. Akhtar, M. A. Lampi, S. Tripuranthakam, D. G. Dixon, and B. M. Greenberg Similar Stress Responses are Elicited by Copper and Ultraviolet Radiation in the Aquatic Plant Lemna gibba: Implication of Reactive Oxygen Species as Common Signals Plant Cell Physiol., December 15, 2003; 44(12): 1320 - 1329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mino, K. Maekawa, K.'i. Ogawa, H. Yamagishi, and M. Inoue Cell Death Processes during Expression of Hybrid Lethality in Interspecific F1 Hybrid between Nicotiana gossei Domin and Nicotiana tabacum Plant Physiology, December 1, 2002; 130(4): 1776 - 1787. [Abstract] [Full Text] [PDF]
|
|