Journal of Experimental Botany, Vol 49, 1705-1714, Copyright © 1998 by Oxford University Press
E Gonzalez, P Aparicio-Tejo, A Gordon, F Minchin, M Royuela and C Arrese-Igor
Two experiments were carried out to investigate the effects of
water-deficit stress on carbon and nitrogen metabolism of Pisum
sativum nodules. In the first experiment, leaf
ARTICLES
Water-deficit effects on carbon and nitrogen metabolism of pea nodules
Department of Molecular Biology, Wageningen Agricultural University, Dreijenlaan 3, NL-6703 HA Wageningen, The Netherlands; Departamento de Ciencias del Medio Natural, Universidad Publica de Navarra, Campus de Arrosadia, E-31006 Pamplona, Spain; Institute for Glassland and Environment Research, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK; ; Corresponding author
w was
allowed to reach -1.0 MPa over a period of 14 d whilst in the second
experiment -1.5 MPa was reached during the same time period. Nodule
activities of phosphoenol pyruvate carboxylase, glutamine synthetase,
alkaline invertase, pyruvate decarboxylase, alcohol dehdyrogenase, uridine
pyro-phosphorylase, and malate dehydrogenase activities were not affected
by water-deficit stress. In the first experiment (-1.0 MPa), sucrose
synthase (SS), an enzyme which hydrolyses sucrose to support nodule
metabolism, declined by 50% in activity and about 25% in content, according
to Western immunoblot data. In the second experiment (-1.5 MPa), SS
activity decreased by 75% together with glutamate synthase and aspartate
aminotransferase which declined by 60% and 40%, respectively. Coincident
with the decline of these activities, a dramatic increase in the nodule
content of sucrose and a slight increase in the levels of total free amino
acids were found. It has been recently suggested that the decline in SS
activity and, therefore, a reduced potential to metabolize sucrose may be
an important factor contributing to the overall response of soybean nodules
to water stress. These results suggest that this observation may be also
correct for temperate legumes with indeterminate nodules. However, in this
latter case, the activity of some enzymes involved in nitrogen assimilation
(glutamate synthase and aspartate aminotransferase) were also affected by
water-deficit stress).Key words: Pisum sativum, water
stress, nitrogen metabolism, nodule metabolism, pea, sucrose synthase.
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