Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Kenton, P.
Right arrow Articles by Draper, J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kenton, P.
Right arrow Articles by Draper, J.
Agricola
Right arrow Articles by Kenton, P.
Right arrow Articles by Draper, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol 50, 1331-1341, Copyright © 1999 by Oxford University Press

ARTICLES

A requirement for calcium and protein phosphatase in the jasmonate-induced increase in tobacco leaf acid phosphatase specific activity

P Kenton, L Mur and J Draper
Institute of Biological Sciences, University of Wales, Aberystwyth, UK; Corresponding author e-mail: jhd@aber.ac.uk

Jasmonic acid (JA) is an established wound signal and also plays a role in plant-pathogen interactions. Application of JA to tobacco leaf explants, tobacco seedlings or to intact leaves via the petiole resulted in an increase in the specific activity of acid phosphatase (AP) and a reduction in overall protein content. Similar changes in AP activity were observed in wounded tissue and in tissue undergoing a hypersensitive response (HR) following infiltration with a virulent bacteria. The AP activity increase was restricted to wounded tissue and HR lesions and was absent from unwounded or uninfiltrated tissue on the same leaf. The JA response (AP increase and protein loss combined) was investigated pharmacologically. Co-incubation with EGTA, ruthenium red, LaCl3 and ()-verapamil blocked the JA response suggesting a requirement for Ca2+ mobilization. Similarly, okadaic acid, cantharidin and microcystin LR abrogated the response to JA implicating a protein phosphatase in the JA signal transduction mechanism(s). No evidence was found for kinase involvement as a mediator of JA signalling in this system.Key words: Jasmonate, signalling, wounding, pathogenesis.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
X. Pang, T. Halaly, O. Crane, T. Keilin, A. Keren-Keiserman, A. Ogrodovitch, D. Galbraith, and E. Or
Involvement of calcium signalling in dormancy release of grape buds
J. Exp. Bot., September 1, 2007; 58(12): 3249 - 3262.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Lannoo, G. Vandenborre, O. Miersch, G. Smagghe, C. Wasternack, W. J. Peumans, and E. J. M. Van Damme
The Jasmonate-Induced Expression of the Nicotiana tabacum Leaf Lectin
Plant Cell Physiol., August 1, 2007; 48(8): 1207 - 1218.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. H. Evans
Modulation of Guard Cell Plasma Membrane Potassium Currents by Methyl Jasmonate
Plant Physiology, January 1, 2003; 131(1): 8 - 11.
[Full Text] [PDF]

Home page
 J Exp BotHome page
J.L. Jakobek and P.B. Lindgren
Expression of a bean acid phosphatase cDNA is correlated with disease resistance
J. Exp. Bot., February 1, 2002; 53(367): 387 - 389.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Leon, E. Rojo, and J. J. Sanchez-Serrano
Wound signalling in plants
J. Exp. Bot., January 1, 2001; 52(354): 1 - 9.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.