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Journal of Experimental Botany, Vol 50, 1547-1552, Copyright © 1999 by Oxford University Press

ARTICLES

Cytoplasmic magnesium regulates the fast activating vacuolar cation channel

L Bruggemann, I Pottosin and G Schonknecht
Molecular Plant Physiology and Biophysics, Botany I, University of Wurzburg, Julius-von-Sachs-Platz 2, D-97082 Wurzburg, Germany; Biomedical Centre, University of Colima, 28047, Colima, Mexico; Corresponding author; Fax: +49 931 888 6158; E-mail: gerald@botanik.uni-wuerzburg.de

Fast activating vacuolar (FV) channels, which are permeable for small monovalent cations, dominate the ion conductance of the vacuolar membrane at physiological Ca2+ concentrations. Here the effect of Mg2+ on FV channels was studied. Patch-clamp measurements were performed on whole barley (Hordeum vulgare) mesophyll vacuoles and on excised tonoplast patches. Free Mg2+ concentrations in the millimolar range inhibited FV channels from the cytosolic and the vacuolar side. Increasing cytosolic free Mg2+ decreased the open probability of FV channels without affecting single channel current amplitudes. The Mg2+ effect showed a bell-shaped voltage-dependence and was most pronounced at voltages between -40 and -60 mV. The dose dependence of the FV channel inhibition by cytosolic Mg2+ could be described by a simple Michaelis-Menten type of binding with Kd values of 10 and 35 M at -60 mV and +100 mV, respectively.Keywords: Hordeum vulgare, patch-clamp, vacuolar membrane, cation channel, magnesium block.
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