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Journal of Experimental Botany, Vol 50, 1553-1559, Copyright © 1999 by Oxford University Press

ARTICLES

Characterization of the elicitor-induced biosynthesis and secretion of genistein from roots of Lupinus luteus L

R Kneer, A Poulev, A Olesinski and I Raskin
Biotech Center, Foran Hall, Cook College, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901-8520, USA; Corresponding author; Fax: +1 732 932 6535; E-mail: raskin@Aesop.rutgers.edu

Genistein is a multi-functional isoflavonoid naturally secreted from roots of hydroponically grown legume plants. Roots of hydroponically cultivated yellow lupin (Lupinus luteus L.) plants, transferred into water secreted minor amounts of genistein (about 5 g g-1 fr. wt.). Secretion of genistein from L. luteus roots (rhizosecretion) was stimulated dramatically to over 100 g g-1 root fresh weight by soluble chitosan, salicylic acid (SA) and potassium cyanide (KCN) supplied at 0.12% (w/v), 800 M and 400 M, respectively. Other identified elicitors caused a smaller induction of genistein rhizosecretion. Increased levels of genistein in root exudates corresponded to greater amounts of genistein in root tissue. Elicitor-induced rhizosecretion of genistein was based on de novo synthesis and was inhibited by glyphosate and other less specific metabolic inhibitors. Except for NaF:AICI3, all tested elicitors of genistein rhizosecretion produced a distinct bell-shaped dose-response curve. Most of the elicitor-induced rhizosecretion of genistein occurred during the first day, followed by a gradual decline. Further addition of elicitor treatments had little effect of genistein rhizosecretion, indicating that the induction of genistein rhizosecretion by the identified elicitors is a once only event.Keywords: Genistein, isoflavonoids, Lupinus luteus, elicitation, exudation.
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