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Journal of Experimental Botany, Vol 50, 1561-1566, Copyright © 1999 by Oxford University Press


ARTICLES

The expression patter of the Arabidopsis ACC synthase gene 1 during rosette leaf development

J Smalle, M Haegman, J Mertens, J Vangronsveld, M Van Montagu and D Van Der Straeten
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium; Limburgs Universitair Centrum, Departement SBG, Universitair Campus, Gebouw D, B-3590 Diepenbeek, Belgium; Corresponding author; Fax: +32 9 2645349; E-mail: dostr@gengenp.rug.ac.be; Present address: Department of Wisconsin, 1575 Linden Drive, Madison, WI 53706, USA

In Arabidopsis thaliana (L.) Heynh., 1-aminocyclopropane-1-carboxylate (ACC) synthase, an enzyme of the ethylene biosynthesis pathway, is encoded by a gene family that contains at least five members. The AT-ACS1 gene is regulated in a tissue-specific manner and is induced during early development. Expression of AT-ACS1 during rosette leaf development was analysed in further detail by using a competitive reverse transcriptase PCR method to monitor transcript levels in individual leaves at different stages of rosette development. AT-ACS1 expression was the highest at leaf emergence and decreased sharply in rapidly expanding leaves, to increase again after the leaf expansion phase. Leaf emergence was also accompanied by an increase in ethylene production. AT-ACS1 transcript levels in rosette leaves were not influenced by the transition from the vegetative to the reproductive phase.Keywords: ACC, Arabidopsis thaliana, ethylene, leaf development.
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