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Journal of Experimental Botany, Vol. 51, No. 343, pp. 305-307, February 2000
© 2000 Oxford University Press

Methods Papers

The monoclonal antibody MAC252 does not react with the (-) enantiomer of abscisic acid

Philippe Barrieu and Thierry Simonneau1

Ecophysiologie des Plantes sous Stress Environnementaux, INRA, 2 place Viala, F-34060 Montepellier cedex 1, France

Abstract

An RIA procedure has been developed for ABA quantification using MAC62, a monoclonal antibody raised against (+)-cis, trans-ABA. This widely used method now relies on MAC252, a recloned version of the exhausted MAC62. Recently, it has been suggested that MAC252 was not able to discriminate between the (+) and (-) enantiomers of ABA. As this can be misleading when interpreting RIA results, it has been carefully examined here whether MAC252 reacts with (-)-ABA. MAC252 exhibited negligible cross-reactivity with (-)-ABA, which was confirmed with commercial mixtures of ABA isomers. It is concluded that the RIA protocol can continue to be used with MAC252 as it was with MAC62.

Key words: RIA, MAC252, ABA isomers


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