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Journal of Experimental Botany, Vol. 51, No. 345, pp. 807-815, April 2000
© 2000 Oxford University Press

Limiting CO2 levels induce a blue light-dependent HCO3- uptake system in Monoraphidium braunii

Nuria Giráldez, Pedro J. Aparicio and Miguel A. Quiñones1

Centro de Investigaciones Biológicas, CSIC, Velázquez 144, E-28006 Madrid, Spain

The in situ photoactivation of an uptake system in the green alga Monoraphidium braunii requires the irradiation of the cell suspensions with short wavelength radiation (blue, UVA and/or UVC). Plasma membrane ATPase inhibitors block the uptake of this monovalent anion at pH 9. M. braunii cells grown in high CO2lack an uptake system in their plasma membrane, but those grown in low CO2can take up this anion at high rates. Cells grown in high CO2, transferred to CO2-limiting conditions in the light, start taking up in 30 min, although they take 90 min to reach maximum rates of transport. Therefore, this induction process seems to be triggered by low external CO2 concentration. In fact, increasing or decreasing the external concentration does not induce the uptake system and only a decrease in CO2 concentration in the medium triggers the induction process. The appearance of the transport activity is sensitive to cycloheximide, indicating that cytoplasmic protein biosynthesis is necessary for the induction of the uptake system. Photosynthetically active radiation, but not particularly blue light, is essential for induction of the uptake system to occur and the inhibition of photosynthesis by DCMU blocks it. From these results it can be inferred that when M. braunii cells detect a drop in CO2 concentration, they induce a blue light-dependent uptake system.

Key words: Blue light, transport, CO2 concentration, Monoraphidium braunii, photosynthesis, protein biosynthesis.


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C. Mora, F. G. Witt, P. J. Aparicio, and M. A. Quinones
Independent induction of two blue light-dependent monovalent anion transport systems in the plasma membrane of Monoraphidium braunii
J. Exp. Bot., September 1, 2002; 53(376): 1909 - 1918.
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