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Journal of Experimental Botany, Vol. 51, No. 349, pp. 1349-1356, August 2000
© 2000 Oxford University Press

Original Papers

Short-term nitrogen-induced modulation of phosphoenolpyruvate carboxylase in tobacco and maize leaves

E.H. Murchie1,3, S. Ferrario-Méry1, M-H. Valadier1 and C.H. Foyer2,4

1 Laboratoire du Métabolisme et de la Nutrition des Plantes, INRA, Route de St Cyr, 78026 Versailles Cedex, France
2 Department of Biochemistry and Physiology, IACR-Rothamstead, Harpenden, Hertfordshire AL5 2JQ, UK

Untransformed maize and tobacco plants and tobacco plants constitutively expressing nitrate reductase were grown with sufficient to support maximal growth. Four days prior to treatment the tobacco plants were deprived of nitrogen. Excised maize leaves and tobacco leaf discs were fed with either 40 mM KNO3 or 40 mM KCl (control) in the light. Phosphoenolpyruvate (PEP) carboxylase (Case) activity was measured at 0.3 mM and 3 mM PEP. The light- induced increase in PEPCase Vmax was greater in maize than tobacco. Furthermore light decreased malate sensitivity in maize (which was N-replete) but not in N-deficient tobacco. treatment increased PEPCase Vmax values in both species and decreased the sensitivity to inhibition by malate, but effects of were much more pronounced in tobacco than maize. PEPCase kinase activity was, however, greater in maize leaves fed than in the Cl--treated controls, suggesting that it is responsive to leaf nitrogen supply. A correlation between foliar glutamine content and PEPCase activity was observed. It is concluded that PEPCase is sensitive to N metabolites which favour increased flow through the anapleurotic pathway in both C3 and C4 plants.

Key words: Anapleurotic pathway, nitrogen assimilation, protein kinase, C3 PEPCase, C4 PEPCase.


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