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Journal of Experimental Botany, Vol. 52, No. 355, pp. 277-283, February 2001
© 2001 Oxford University Press

Original Papers

Rapid N transport to pods and seeds in N-deficient soybean plants

Norikuni Ohtake1,5, Takashi Sato1, Hiroyuki Fujikake1, Kuni Sueyoshi1, Takuji Ohyama1, Noriko-Shigeta Ishioka2, Satoshi Watanabe2, Akihiko Osa2, Toshiaki Sekine2, Shinpei Matsuhashi3, Taketo Ito3, Chizuko Mizuniwa3, Tamikazu Kume3, Shoji Hashimoto3, Hiroshi Uchida4 and Astunori Tsuji4

1 Faculty of Agriculture, University of Niigata, 2-8050 Ikarashi, Niigata-city, Niigata 951-2181, Japan
2 Department of Radioisotopes, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan
3 Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan
4 Central Research Laboratory, Hamamatsu Photonics Co., Shizuoka 434-0041, Japan

Non-nodulated soybean (Glycine max (L.) Merr.) plants were cultivated hydroponically under N-sufficient (5 mM NaNO3) or N-deficient (0.5 mM NaNO3) conditions. 13N- or 15N- labelled nitrate was fed to the cut end of the stems, and the accumulation of nitrate-derived N in the pods, nodes and stems was compared. Real-time images of 13N distribution in stems, petioles and pods were obtained using a Positron Emitting Tracer Imaging System for a period of 40 min. The results indicated that the radioactivity in the pods of N-deficient plants was about 10 times higher than that of N-sufficient plants, although radioactivity in the stems and nodes of N-deficient versus N-sufficient plants was not different. A similar result was obtained by supplying 15 to cut soybean shoots for 1 h. The fact that the N translocation into the pods from fed to the stem base was much faster in N-deficient plants may be due to the strong sink activity of the pods in N-deficient plants. Alternatively, the redistribution of N from the leaves to the pods via the phloem may be accelerated in N-deficient plants. The temporal accumulation of 13 in nodes was suggested in both N-sufficient and N-deficient plants. In one 13 pulse-chase experiment, radioactivity in the stem declined rapidly after transferring the shoot from the 13 solution to non-labelled NO3; in contrast, the radioactivity in the node declined minimally during the same time period.

Key words: Soybean, nitrogen deficient, nitrogen requirement, nitrate translocation, PETIS.


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H. Fujikake, A. Yamazaki, N. Ohtake, K. Sueyoshi, S. Matsuhashi, T. Ito, C. Mizuniwa, T. Kume, S. Hashimoto, N.-S. Ishioka, et al.
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