Journal of Experimental Botany, Vol. 52, No. 356, pp. 577-590,
April 2001
© 2001 Oxford University Press
Compartmentation of photosynthesis in cells and tissues of C4 plants
1 School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA
2 Department of Anatomy and Morphology, V. L. Komarov Botanical Institute of Russian Academy of Sciences, Prof. Popov Street 2, 197376 St Petersburg, Russia
3 Department of Plant Physiology, Ural State University, Lenin Avenue 51, 620083 Ekaterinburg, Russia
4 Centro de Estudios Fotosintéticos y Bioquímicos, (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina
Critical to defining photosynthesis in C4 plants is understanding the intercellular and intracellular compartmentation of enzymes between mesophyll and bundle sheath cells in the leaf. This includes enzymes of the C4 cycle (including three subtypes), the C3 pathway and photorespiration. The current state of knowledge of this compartmentation is a consequence of the development and application of different techniques over the past three decades. Initial studies led to some alternative hypotheses on the mechanism of C4 photosynthesis, and some controversy over the compartmentation of enzymes. The development of methods for separating mesophyll and bundle sheath cells provided convincing evidence on intercellular compartmentation of the key components of the C4 pathway. Studies on the intracellular compartmentation of enzymes between organelles and the cytosol were facilitated by the isolation of mesophyll and bundle sheath protoplasts, which can be fractionated gently while maintaining organelle integrity. Now, the ability to determine localization of photosynthetic enzymes conclusively, through in situ immunolocalization by confocal light microscopy and transmission electron microscopy, is providing further insight into the mechanism of C4 photosynthesis and its evolution. Currently, immunological, ultrastructural and cytochemical studies are revealing relationships between anatomical arrangements and photosynthetic mechanisms which are probably related to environmental factors associated with evolution of these plants. This includes interesting variations in the C4 syndrome in leaves and cotyledons of species in the tribe Salsoleae of the family Chenopodiaceae, in relation to evolution and ecology. Thus, analysis of structure–function relationships using modern techniques is a very powerful approach to understanding evolution and regulation of the photosynthetic carbon reduction mechanisms.
Key words: Anatomy, C4 plants, chloroplasts, gene expression, immunolocalization, photosynthetic enzymes, ultrastructure.
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