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Journal of Experimental Botany, Vol. 52, No. 358, pp. 901-910, May 1, 2001
© 2001 Oxford University Press

Original Papers

In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen-inducible PR 10 promoter

P. Coutos-Thévenot1,4, B. Poinssot1, A. Bonomelli1, H. Yean1, C. Breda2, D. Buffard2, R. Esnault2, R. Hain3 and M. Boulay1

1 LVMH-Recherche: Moët et Chandon, 20 Avenue de Champagne, BP 140 51333 Epernay Cédex, France
2 Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 92198 Gif sur Yvette Cédex, France
3 Bayer AG, PF-E/Fu, Institut für Biotechnologie, D-5090 Leverkusen 1, Germany

Resveratrol is a major phytoalexin in grapevine but its synthesis in response to phytopathogen attack decreases with grape berry ripening. A chimeric gene combining an alfalfa PR 10 promoter and Vst1 (Vitis stilbene synthase 1) gene was introduced into the genome of 41B rootstock. Transgenic plants were analysed for resveratrol production in leaves infected with Botrytis using an in vitro test. Among the 50 transgenic lines analysed, some exhibited a production lower than the non-transgenic control, but others accumulated resveratrol from 5–100-fold. Moreover, in the latter clones, symptoms were highly reduced in response to infection. These results were a good indication that the combination of a pathogen-inducible promoter and a defence gene may increase tolerance against fungi in grapevine. The efficacy of this approach should be further tested by experiments conducted in the vineyard.

Key words: Grapevine, Botrytis, stilbene synthase, inducible promoter, ‘PR’ proteins.


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