Journal of Experimental Botany, Vol. 52, No. 361, pp. 1635-1645,
August 1, 2001
© 2001 Oxford University Press
Original Papers |
ß-Galactosidases with a lectin-like domain are expressed in strawberry1
Dipartimento di Biologia, Università di Padova, Viale G. Colombo 3, I-35121 Padova, Italy
Strawberry fruits (Fragariaxananassa Duch.) undergo a marked softening during their ripening, and the process is accompanied by a release of free sugars with galactose among them. In this work total ß-galactosidase activity was measured in cell wall proteins from strawberry fruits at different developmental stages. Three full-length cDNAs (Faßgal1, Faßgal2 and Faßgal3, respectively) encoding different ß-galactosidases (EC 3.2.1.23) were isolated from a library representing red fruit transcripts. All of them could be detected both in fruits and in vegetative tissues. However, only Faßgal1 showed an increasing expression during the ripening stages up to a maximum in the red fruits, while the other two (Faßgal2 and Faßgal3) were mostly found in green fruits and became barely detectable during ripening proper. The three ß-galactosidase-encoding cDNAs were expressed in the yeast Pichia pastoris, and it was thus possible to demonstrate that each of them encode a ß-galactosidase. The expression of the three ß-galactosidase genes appears to be down-regulated by auxin, as already observed for other ripening-related genes of the non-climacteric strawberry. An unusual characteristic of two strawberry ß-galactosidases (Faßgal1 and Faßgal2) is that at the C-terminus of the enzymes a domain is found which is structurally related to known animal peptides with a sugar-binding ability.
Key words: Fruit ripening, ß-galactosidase, lectin-like domain, strawberry.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Santiago-Domenech, S. Jimenez-Bemudez, A. J. Matas, J. K. C. Rose, J. Munoz-Blanco, J. A. Mercado, and M. A. Quesada Antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening J. Exp. Bot., July 1, 2008; 59(10): 2769 - 2779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Raab, J. A. Lopez-Raez, D. Klein, J. L. Caballero, E. Moyano, W. Schwab, and J. Munoz-Blanco FaQR, Required for the Biosynthesis of the Strawberry Flavor Compound 4-Hydroxy-2,5-Dimethyl-3(2H)-Furanone, Encodes an Enone Oxidoreductase PLANT CELL, April 1, 2006; 18(4): 1023 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Trainotti, A. Pavanello, and G. Casadoro Different ethylene receptors show an increased expression during the ripening of strawberries: does such an increment imply a role for ethylene in the ripening of these non-climacteric fruits? J. Exp. Bot., August 1, 2005; 56(418): 2037 - 2046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kotake, S. Dina, T. Konishi, S. Kaneko, K. Igarashi, M. Samejima, Y. Watanabe, K. Kimura, and Y. Tsumuraya Molecular Cloning of a {beta}-Galactosidase from Radish That Specifically Hydrolyzes {beta}-(1->3)- and {beta}-(1->6)-Galactosyl Residues of Arabinogalactan Protein Plant Physiology, July 1, 2005; 138(3): 1563 - 1576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wu and J. K. Burns A {beta}-galactosidase gene is expressed during mature fruit abscission of 'Valencia' orange (Citrus sinensis) J. Exp. Bot., July 1, 2004; 55(402): 1483 - 1490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Castillejo, J. I. de la Fuente, P. Iannetta, M. A. Botella, and V. Valpuesta Pectin esterase gene family in strawberry fruit: study of FaPE1, a ripening-specific isoform J. Exp. Bot., April 1, 2004; 55(398): 909 - 918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aharoni and A. P. O'Connell Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays J. Exp. Bot., October 1, 2002; 53(377): 2073 - 2087. [Abstract] [Full Text] [PDF]
|
|