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Journal of Experimental Botany, Vol. 52, No. 362, pp. 1753-1759, September 1, 2001
© 2001 Oxford University Press

Original Papers

Antisense-transformation reveals novel roles for class I ß-1,3-glucanase in tobacco seed after-ripening and photodormancy

Gerhard Leubner-Metzger1,2,3 and Frederick Meins, Jr1

1 Friedrich Miescher-Institute, Maulbeerstrasse 66, CH-4058 Basel, Switzerland
2 Institut für Biologie II, Albert-Ludwigs-Universität, Schänzlestr. 1, D-79104 Freiburg, Germany

Little is known about the molecular basis for seed dormancy, after-ripening, and radicle emergence through the covering layers during germination. In tobacco, endosperm rupture occurs after testa rupture and is the limiting step in seed germination. Class I ß-1,3-glucanase (ßGLU I), which is induced in the micropylar endosperm just prior to its penetration by the radicle, is believed to help weaken the endosperm wall. Evidence is pesented here for a second site of ßGLU I action during after-ripening. Tobacco plants were transformed with antisense ßGLU I constructs with promoters thought to direct endosperm-specific expression. Unexpectedly, these transformants were unaffected in endosperm rupture and did not exhibit reduced ßGLU I expression during germination. Nevertheless, antisense ßGLU I transformation delayed the onset of testa rupture in light-imbibed, after-ripened seeds and inhibited the after-ripening-mediated release of photodormancy. It is proposed that ßGLU I expression in the dry seed contributes to the after-ripening-mediated release of seed dormancy.

Key words: Abscisic acid, after-ripening, coat-enhanced seed dormancy, endosperm-limited seed germination, ß-1,3-glucanase, sense- and antisense transformation.


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