Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Siefritz, F.
Right arrow Articles by Kaldenhoff, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Siefritz, F.
Right arrow Articles by Kaldenhoff, R.
Agricola
Right arrow Articles by Siefritz, F.
Right arrow Articles by Kaldenhoff, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 52, No. 363, pp. 1953-1957, October 1, 2001
© 2001 Oxford University Press

Original Papers

The tobacco plasma membrane aquaporin NtAQP1

Franka Siefritz, Alexander Biela, Martin Eckert, Beate Otto, Norbert Uehlein and Ralf Kaldenhoff1

Universität Würzburg, Molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs-Platz 2, D-97082 Würzburg, Germany

This paper gives a summary of a project to characterize a tobacco aquaporin. The cDNA and gene, including the 5' upstream region, for the tobacco aquaporin NtAQP1 has been isolated and the encoded protein characterized. The significance of promoter regions for an abscisic acid- and gibberellic acid-induced gene expression could be restricted to a region between -1450 and -1112 upstream of the transcription start point by transient transformation of a bicistronic vector into tobacco protoplasts. NtAQP1 expression in tobacco plants was found to be elevated in flowers, stems and roots. In roots, the protein was detected close to xylem vessels in pitch-like structures. Studies with a NtAQP1-GFP fusion indicated a plasma membrane location. For a functional analysis, the cDNA was expressed in Xenopus oocytes. NtAQP1 was found to be a heavy metal-insensitive aquaporin with additional permeability for glycerol. Mutation of a threonine at position 233 to a cysteine transformed NtAQP1 into a heavy metal-sensitive aquaporin.

Key words: Aquaporin, gene expression, Nicotiana tabaccum.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
H. Evelin, R. Kapoor, and B. Giri
Arbuscular mycorrhizal fungi in alleviation of salt stress: a review
Ann. Bot., October 8, 2009; (2009) mcp251v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Ma, J. Xue, Y. Li, X. Liu, F. Dai, W. Jia, Y. Luo, and J. Gao
Rh-PIP2;1, a Rose Aquaporin Gene, Is Involved in Ethylene-Regulated Petal Expansion
Plant Physiology, October 1, 2008; 148(2): 894 - 907.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Martre, R. Morillon, F. Barrieu, G. B. North, P. S. Nobel, and M. J. Chrispeels
Plasma Membrane Aquaporins Play a Significant Role during Recovery from Water Deficit
Plant Physiology, December 1, 2002; 130(4): 2101 - 2110.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.