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Journal of Experimental Botany, Vol. 52, No. 365, pp. 2367-2374, December 1, 2001
© 2001 Oxford University Press

Original Papers

Expression of a cold-responsive Lt-Cor gene and development of freezing tolerance during cold acclimation in wheat (Triticum aestivum L.)

R. Ohno, S. Takumi and C. Nakamura1

Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, and Division of Life Science, Graduate School of Science and Technology, Kobe University, 1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan

Time-courses of the development of freezing tolerance and the expression of a cold-responsive gene wlt10 were monitored during cold acclimation in wheat (Triticum aestivum L.). Bioassay showed that cold acclimation conferred much higher freezing tolerance on a winter cultivar than a spring cultivar. Northern blot analysis showed that the expression of wlt10 encoding a novel wheat member of a cereal-specific LT-COR protein family was specifically induced by low temperature. A freezing-tolerant winter cultivar accumulated the mRNA more rapidly and for a longer period than a susceptible spring cultivar. The increase in the amount of mRNA was temporary but the peak occurred at the time when the maximum level of freezing tolerance was attained. The mRNA accumulated more in the leaves than in the roots, and different light/dark regimes modulated the level of mRNA accumulation. Genomic Southern blot analyses using the nulli-tetrasomic series showed that the wlt10 homologues were located on the homologous group 2 chromosomes.

Key words: Cold acclimation, freezing tolerance, low temperature-responsive (Lt) gene, cold-responsive (Cor) gene, wheat (Triticum aestivum L.).


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