Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by McCartney, L.
Right arrow Articles by Knox, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McCartney, L.
Right arrow Articles by Knox, J. P.
Agricola
Right arrow Articles by McCartney, L.
Right arrow Articles by Knox, J. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 53, No. 369, pp. 707-713, April 1, 2002
© 2002 Oxford University Press

Original Papers

Regulation of pectic polysaccharide domains in relation to cell development and cell properties in the pea testa

Lesley McCartney and J. Paul Knox1

Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK

The occurrence of pectic polysaccharide epitopes in cells and tissues of the pea testa during late stages of seed development have been examined in relation to anatomy and cell properties. Homogalacturonan, in a highly methyl-esterified form, was present throughout late development in all pea testa cell walls, including the thickened cell walls of the outer macrosclereid layer. Two epitopes, characteristic of the side-chains of the rhamnogalacturonan-I domain of pectic polysaccharides, occurred in restricted and separate cell layers of the pea testa. A (1->4)-ß-D-galactan epitope was restricted to regions of the outer cell wall of the testa and to inner regions of the macrosclereid layer at 20 DAA and was absent from the osteosclereid and parenchyma cell walls. By 25 DAA the (1->4)-ß-D-galactan epitope occurred only in the outer epidermal cell walls. A (1->5)-{alpha}-L-arabinan epitope was also dependent on the developmental stage of the seed and was found with greatest abundance in the walls of the inner parenchyma cells. Cell separation studies indicated that, although calcium cross-links were involved in the maintenance of the link between the macrosclereid layer and proximal cell layers, most cell-to-cell adhesion in the testa was not due to calcium- or ester-based bonds.

Key words: Cell properties, pectin, pectic polysaccharides, Pisum sativum L., testa.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. A. Arsovski, T. M. Popma, G. W. Haughn, N. C. Carpita, M. C. McCann, and T. L. Western
AtBXL1 Encodes a Bifunctional {beta}-D-Xylosidase/{alpha}-L-Arabinofuranosidase Required for Pectic Arabinan Modification in Arabidopsis Mucilage Secretory Cells
Plant Physiology, July 1, 2009; 150(3): 1219 - 1234.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Guillon, S. Philippe, B. Bouchet, M.-F. Devaux, P. Frasse, B. Jones, M. Bouzayen, and M. Lahaye
Down-regulation of an Auxin Response Factor in the tomato induces modification of fine pectin structure and tissue architecture
J. Exp. Bot., February 10, 2008; (2008) erm323v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. H. Dean, H. Zheng, J. Tewari, J. Huang, D. S. Young, Y. T. Hwang, T. L. Western, N. C. Carpita, M. C. McCann, S. D. Mansfield, et al.
The Arabidopsis MUM2 Gene Encodes a {beta}-Galactosidase Required for the Production of Seed Coat Mucilage with Correct Hydration Properties
PLANT CELL, December 1, 2007; 19(12): 4007 - 4021.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. Minic, C.-T. Do, C. Rihouey, H. Morin, P. Lerouge, and L. Jouanin
Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis
J. Exp. Bot., July 1, 2006; 57(10): 2339 - 2351.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Leboeuf, F. Guillon, S. Thoiron, and M. Lahaye
Biochemical and immunohistochemical analysis of pectic polysaccharides in the cell walls of Arabidopsis mutant QUASIMODO 1 suspension-cultured cells: implications for cell adhesion
J. Exp. Bot., December 1, 2005; 56(422): 3171 - 3182.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. Konno, T. Nakato, S. Nakashima, and K. Katoh
Lygodium japonicum fern accumulates copper in the cell wall pectin
J. Exp. Bot., July 1, 2005; 56(417): 1923 - 1931.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Leboeuf, S. Thoiron, and M. Lahaye
Physico-chemical characteristics of cell walls from Arabidopsis thaliana microcalli showing different adhesion strengths
J. Exp. Bot., September 1, 2004; 55(405): 2087 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. M. Fulton and C. S. Cobbett
Two {alpha}-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development
J. Exp. Bot., November 1, 2003; 54(392): 2467 - 2477.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn
Distribution of Fucose-Containing Xyloglucans in Cell Walls of the mur1 Mutant of Arabidopsis
Plant Physiology, April 1, 2003; 131(4): 1602 - 1612.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.