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Journal of Experimental Botany, Vol. 53, No. 371, pp. 1219-1221, May 2002
© 2002 Oxford University Press


Gene Note

Cloning and expression of an ABSCISIC ACID-INSENSITIVE 3 (ABI3) gene homologue of yellow-cedar (Chamaecyparis nootkatensis)

Galina Lazarova, Ying Zeng and Allison R. Kermode 1

Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada V5A 1S6

Abstract

A homologue of the ABI3 gene was isolated from the conifer species, Chamaecyparis nootkatensis. The deduced protein of 794 amino acids exhibited sequence similarity to other VP1/ABI3 proteins within four regions. Expression occurs exclusively in seeds, with no detectable mRNA in leaves and roots. Unlike the homologues of angiosperms, CnABI3 may be encoded by more than one gene.

Key words: Abscisic acid, ABI3, seed dormancy, yellow-cedar.


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