Journal of Experimental Botany, Vol. 53, No. 376, pp. 1847-1856, September 1, 2002
© 2002 Oxford University Press
Cell and Molecular Biology, Biochemistry and Molecular Physiology |
Stimulation of glycolysis in anaerobic elongation of pondweed (Potamogeton distinctus) turions
Received 28 January 2002; Accepted 9 May 2002
Department of Developmental Biology and Neuroscience, Graduate School of Life Sciences, Tohoku University, 980-8578 Sendai, Japan
1 To whom correspondence should be addressed. Fax: +81 22 217 734. E-mail: kimiharu{at}mail.cc.tohoku.ac.jp
Stem segments prepared from pondweed (Potamo geton distinctus A. Benn.) turions (overwintering buds) elongate in anaerobic conditions, whereas there is almost no elongation in air. The anaerobic elongation was accompanied by a decrease in dry weights of stem segments, mainly due to consumption of storage starch in the amyloplasts of stem cells. On the other hand, total contents of amino acids increased in stem segments, in which contents of alanine, valine, leucine, and isoleucine increased, but contents of asparatic acid decreased. Moreover, contents of lactate in stem tissues increased at an early stage of anaerobic incubation. In tracer experiments with 14C-glucose, 14C incorporation into stem tissues in anoxia was only half of that in normoxia. However, conversion of 14C to ethanol occurred exclusively in anoxia. 14C-labelled metabolites were analysed by two-dimensional cellulose thin-layer chromatography. 14C incorporation into sucrose and alanine was significantly increased in anoxia. The activity of alanine aminotransferase was enhanced by anoxia, suggesting that pyruvate is a precursor of alanine synthesis. The results suggest that pondweed turions produce energy necessary for anaerobic elongation by activating conversion of storage starch in the amyloplasts to ethanol, alanine and lactate.
Key words: Key words: Alanine, amyloplast, anoxia, elongation, ethanol, fermentation, glycolysis, lactate, Potamogeton distinctus A. Benn., turion.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Castonguay, G. Thibault, P. Rochette, A. Bertrand, S. Rochefort, and J. Dionne Physiological Responses of Annual Bluegrass and Creeping Bentgrass to Contrasted Levels of O2 and CO2 at Low Temperatures Crop Sci., March 17, 2009; 49(2): 671 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. U. Igamberdiev and R. D. Hill Plant mitochondrial function during anaerobiosis Ann. Bot., January 1, 2009; 103(2): 259 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Limami, G. Glevarec, C. Ricoult, J.-B. Cliquet, and E. Planchet Concerted modulation of alanine and glutamate metabolism in young Medicago truncatula seedlings under hypoxic stress J. Exp. Bot., June 1, 2008; 59(9): 2325 - 2335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Miyashita and A. G. Good Contribution of the GABA shunt to hypoxia-induced alanine accumulation in roots of Arabidopsis thaliana Plant Cell Physiol., January 1, 2008; 49(1): 92 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ricoult, L. O. Echeverria, J.-B. Cliquet, and A. M. Limami Characterization of alanine aminotransferase (AlaAT) multigene family and hypoxic response in young seedlings of the model legume Medicago truncatula J. Exp. Bot., September 1, 2006; 57(12): 3079 - 3089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nakagawa and N. Sakurai A Mutation in At-nMat1a, Which Encodes a Nuclear Gene Having High Similarity to Group II Intron Maturase, Causes Impaired Splicing of Mitochondrial NAD4 Transcript and Altered Carbon Metabolism in Arabidopsis thaliana Plant Cell Physiol., June 1, 2006; 47(6): 772 - 783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. AGARWAL and A. GROVER Isolation and Transcription Profiling of Low-O2 Stress-Associated cDNA Clones from the Flooding-stress-tolerant FR13A Rice Genotype Ann. Bot., October 1, 2005; 96(5): 831 - 844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. HARADA, S. SATOH, T. YOSHIOKA, and K. ISHIZAWA Expression of Sucrose Synthase Genes Involved in Enhanced Elongation of Pondweed (Potamogeton distinctus) Turions under Anoxia Ann. Bot., September 1, 2005; 96(4): 683 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. OOKAWARA, S. SATOH, T. YOSHIOKA, and K. ISHIZAWA Expression of {alpha}-Expansin and Xyloglucan Endotransglucosylase/Hydrolase Genes Associated with Shoot Elongation Enhanced by Anoxia, Ethylene and Carbon Dioxide in Arrowhead (Sagittaria pygmaea Miq.) Tubers Ann. Bot., September 1, 2005; 96(4): 693 - 702. [Abstract] [Full Text] [PDF]
|
|