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JXB Advance Access originally published online on March 3, 2003
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Journal of Experimental Botany, Vol. 54, No. 385, pp. 1133-1141, April 1, 2003
© 2003 Oxford University Press

A novel alpha-helical protein, specific to and highly conserved in plants, is associated with the nuclear matrix fraction

Received 19 August 2002; Accepted 30 November 2002

Annkatrin Rose3,, Frank Gindullis13, and Iris Meier2,

Plant Biotechnology Center and Department of Plant Biology, Ohio State University, Columbus, OH 43210, USA

1 Present address: CellTec Biotechnologie GmbH, Frohmestrasse 110, D-22459 Hamburg, Germany.
2 To whom correspondence should be addressed. Fax: +1 614 292 5379. E-mail: meier.56{at}osu.edu
3 These two authors have contributed equally to this work.

A cDNA for a novel plant protein was isolated from tomato. Nuclear Matrix Protein 1 (NMP1) is a ubiquitously expressed 36 kDa protein, which has no homologues in animals and fungi, but is highly conserved among flowering and non-flowering plants, including gymnosperms, moss, and the liverwort Marchantia polymorpha. NMP1 is predominantly {alpha}-helical with multiple stretches of short amphipathic regions. Cell fractionation, immunofluorescence, and GFP localization experiments showed that NMP1 is located both in the cytoplasm and nucleus and that the nuclear fraction is associated with the nuclear matrix. NMP1 is a candidate for a plant-specific structural protein with a function both in the nucleus and cytoplasm.

Key words: Amphipathic alpha helix, GFP, nuclear matrix, tomato.


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