JXB Advance Access originally published online on May 13, 2003
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Journal of Experimental Botany, Vol. 54, No. 388, pp. 1665-1673,
July 1, 2003
© 2003 Oxford University Press
Role of visible light in the recovery of photosystem II structure and function from ultraviolet-B stress in higher plants
Received 22 October 2002; Accepted 19 March 2003
1 Dipartimento di Biologia, Università di Padova, Italy
2 Dipartimento di Biologia, Universitá di Milano, Italy
3 Dipartimento di Scienze e Tecnologie Avanzate, Università del Piemonte Orientale, ‘Amedeo Avogadro’, Corso Borsalino 54, 15100 Alessandria, Italy
* To whom correspondence should be addressed. Fax: +39 0131 254410. E-mail: roberto.barbato{at}unipmn.it
Abbreviations: DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea; PAGE, polyacrylamide gel electrophoresis; PSII, photosystem II.
The effect of visible light on photosystem II reaction centre D1 protein in plants treated with ultraviolet-B light was studied. It was found that a 20 kDa C-terminal fragment of D1 protein generated during irradiation with ultraviolet-B light was stable when plants were incubated in the dark, but was degraded when plants were incubated in visible light. In this condition the recovery of photosynthetic activity was also observed. Even a low level of white light was sufficient to promote both further degradation of the fragment and recovery of activity. During this phase, the D1 protein is the main synthesized thylakoid polypeptide, indicating that other photosystem II proteins are recycled in the recovery process. Although both degradation of the 20 kDa fragment and resynthesis of D1 are light-dependent phenomena, they are not closely related, as degradation of the 20 kDa fragment may occur even in the absence of D1 synthesis. Comparing chemical and physical factors affecting the formation of the fragment in ultraviolet-B light and its degradation in white light, it was concluded that the formation of the fragment in ultraviolet-B light is a photochemical process, whereas the degradation of the fragment in white light is a protease-mediated process.
Key words: D1 protein, photosynthesis, photosystem II, ultraviolet-B.
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