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Journal of Experimental Botany, Vol. 54, No. 389, pp. 2001-2002, August 1, 2003
© 2003 Oxford University Press

Calcium/calmodulin activation of two divergent glutamate decarboxylases from tobacco*

Received 30 April 2003; Accepted 7 May 2003

Dmytro P. Yevtushenko, Michael D. McLean, Sriyani Peiris, Owen R. Van Cauwenberghe and Barry J. Shelp{dagger},

Department of Plant Agriculture, Biotechnology Division, Bovey Building, University of Guelph, Guelph, Ontario, Canada N1G 2W1

* The nucleotide sequences for NtGAD1 and NtGAD3 were deposited in the GenBank database under the accession numbers AAK18620 and AAK38667, respectively.
{dagger} To whom correspondence should be addressed. Fax: +519 767 0755. E-mail: bshelp{at}uoguelph.ca

Glutamate decarboxylase (GAD, EC 4.1.1.15) catalyses the {alpha}-decarboxylation of glutamate to produce {gamma}-aminobutyrate (GABA). The nucleotide sequences of two divergent GADs (designated GAD1 and GAD3) were isolated from a Nicotiana tabacum L. cv. Samsun NN leaf cDNA library. Open reading frames indicated that GAD1 encodes a polypeptide of 496 amino acids and has greater than 99% identity with known tobacco GADs, whereas GAD3 encodes a polypeptide of 491 amino acids and has about 14% divergence from known tobacco GADs. Genomic DNA analysis suggested that there are at least four tobacco GAD genes, existing in pairs of highly identical genes. An in vitro assay at pH 7.3 revealed that activities of the recombinant proteins, after isolation from Escherichia coli and partial purification by nickel-affinity chromatography, are 57–133 times the control levels in the presence of 0.5 mM calcium and 0.2 µM bovine calmodulin.

Key words: cDNA sequences, {gamma}-aminobutyrate, glutamate decarboxylase, recombinant protein, tobacco.


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