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JXB Advance Access originally published online on September 9, 2003
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Journal of Experimental Botany, Vol. 54, No. 392, pp. 2587-2588, November 1, 2003
© 2003 Oxford University Press

CrMYC1, a Catharanthus roseus elicitor- and jasmonate-responsive bHLH transcription factor that binds the G-box element of the strictosidine synthase gene promoter*

Received 7 March 2003; Accepted 11 July 2003

Guillaume Chatel2, Grégory Montiel1,2, Martial Pré2,3, Johan Memelink3, Martine Thiersault1,2, Benoit Saint-Pierre1,2, Pierre Doireau2 and Pascal Gantet{dagger},1,2

1 Unité sous Contrat reconnue par l’INRA ‘Facteurs de Transcription et Ingénierie Métabolique Végétale’, UFR des Sciences et Techniques et UFR des Sciences Pharmaceutiques, Université de Tours, France
2 EA 2106, Biomolécules et Biotechnologies Végétales, Université de Tours, UFR des Sciences et Techniques, Laboratoire de Physiologie Végétale, Parc de Grandmont, F-37200 Tours, France
3 Institute of Biology, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

* The sequence of the Crmyc1 cDNA is deposited in the GenBank Nucleotide Sequence Database under the accession number AF283506.
{dagger} To whom correspondence should be addressed. Fax: +33 2 47 36 70 42. E-mail: gantet{at}univ-tours.fr

A cDNA encoding a bHLH transcription factor was isolated by the yeast one-hybrid system from a Catharanthus roseus cDNA library using the G-box element of the Strictosidine synthase gene promoter as bait. The corresponding protein (named CrMYC1) was shown to bind specifically to the G-box in yeast. In C. roseus suspension cells CrMYC1 mRNA levels are induced by fungal elicitor and jasmonate suggesting that CrMYC1 may be involved in the regulation of gene expression in response to these signals.

Key words: bHLH, Catharanthus roseus, cMyc, elicitor, G-box, jasmonate, strictosidine synthase, transcription factor.


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