Skip Navigation


JXB Advance Access originally published online on October 16, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/393/2623    most recent
erg292v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Giannino, D.
Right arrow Articles by Mariotti, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Giannino, D.
Right arrow Articles by Mariotti, D.
Agricola
Right arrow Articles by Giannino, D.
Right arrow Articles by Mariotti, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 393, pp. 2623-2633, December 1, 2003
© 2003 Oxford University Press

Isolation and characterization of a maintenance DNA-methyltransferase gene from peach (Prunus persica [L.] Batsch): transcript localization in vegetative and reproductive meristems of triple buds

Received 26 March 2003;; Accepted 29 July 2003

Donato Giannino*,1,3, Giovanni Mele3, Radiana Cozza2, Leonardo Bruno2, Giulio Testone3, Carla Ticconi4, Giovanna Frugis3, Maria Beatrice Bitonti2, Anna Maria Innocenti2 and Domenico Mariotti3

1 Institute of Plant Protection, Section of Bari, National Research Council of Italy (CNR), Via Amendola 165/A, I-70126 Bari, Italy
2 University of Calabria, Department of Ecology, Laboratory of Botany, Ponte P. Bucci, Cubo 6B, 87030, Arcavacata di Rende, Cosenza, Italy
3 Institute of Biology and Agricultural Biotechnology, Section of Rome, National Research Council of Italy (CNR), via Salaria km 29,300, 00016, Monterotondo Scalo, Rome, Italy
4 Department of Vegetable Crops, University of California, One Shields Avenue, Davis, CA 95616, USA

* To whom correspondence should be addressed in Rome. Fax: +39 0690 64492. E-mail: giannino{at}mlib.cnr.it

A cDNA coding for a DNA (cytosine-5)-methyltransferase (METase) was isolated from peach (Prunus persica [L.] Batsch) and the corresponding gene designated as PpMETI. The latter encoded a predicted polypeptide of 1564 amino acid residues and harboured all the functional domains conserved in the maintenance METases group type I. PpMETI was a single copy in the cultivar Chiripa which was used as a model in the present study. Expression analyses revealed that PpMETI transcripts were more abundant in tissues with actively proliferating cells such as apical tips, uncurled leaves, elongating herbaceous stems, and small immature fruits. Peach plants bear bud clusters (triads or triple buds), consisting of two lateral and one central bud with floral and vegetative fates, respectively. PpMETI in situ hybridization was performed in triple buds during their entire developmental cycle. High and low levels of PpMETI transcript were related to burst and quiescence of vegetative growth, respectively. Message localization distinguished lateral from central buds during the meristem switch to the floral phase. In fact, the PpMETI message was abundant in the L1 layer of protruding domes, a morphological trait marking the beginning of floral transition. The PpMETI transcript was also monitored during organ flower formation. Altogether, these data suggest a relationship between DNA replication and PpMETI gene expression.

Key words: Buds with distinct fates, DNA methylase expression, peach.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Testone, L. Bruno, E. Condello, A. Chiappetta, A. Bruno, G. Mele, A. Tartarini, L. Spano, A. M. Innocenti, D. Mariotti, et al.
Peach [Prunus persica (L.) Batsch] KNOPE1, a class 1 KNOX orthologue to Arabidopsis BREVIPEDICELLUS/KNAT1, is misexpressed during hyperplasia of leaf curl disease
J. Exp. Bot., February 5, 2008; (2008) erm317v2.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.