Skip Navigation


JXB Advance Access originally published online on December 12, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/395/199    most recent
erh027v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Mills, L. N.
Right arrow Articles by Hetherington, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mills, L. N.
Right arrow Articles by Hetherington, A. M.
Agricola
Right arrow Articles by Mills, L. N.
Right arrow Articles by Hetherington, A. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 395, pp. 199-204, January 1, 2004
© 2004 Oxford University Press

Signalling in Abiotic Stress

The effects of manipulating phospholipase C on guard cell ABA-signalling

Received 12 May 2003; Accepted 8 October 2003

Lewis N. Mills1, Lee Hunt2, Calum P. Leckie3, Fiona L. Aitken2, Mark Wentworth2, Martin R. McAinsh1, Julie E. Gray2 and Alistair M. Hetherington1,*

1 Department of Biological Sciences, Lancaster Environment Centre, University of Lancaster, Lancaster LA1 4YQ, UK
2 Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
3 School of Cell and Molecular Biosciences, Medical School, University of Newcastle Upon Tyne, Framlington Place, Newcastle Upon Tyne NE2 4HH, UK

* To whom correspondence should be addressed. Fax: +44 (0)1524 843854. E-mail: A.Hetherington{at}lancaster.ac.uk

Studies using stably transformed tobacco plants containing very low levels of PI-PLC in their guard cells show that this enzyme plays a role in the events associated with the inhibition of stomatal opening by ABA, but not in the cellular reactions that are responsible for ABA-induced stomatal closure. However, Commelina communis guard cells microinjected with the InsP3 antagonist, heparin, fail to close on addition of ABA. There are three possible explanations for this apparent data mismatch. The differences may be indicative of species-specific signalling pathways, the presence of a PI-PLC isoform(s) that is not down-regulated in these transgenic lines and/or they may reflect differences between short-term (acute) administration of an inhibitor and long-term (chronic) effects of gene manipulation. It is possible that the guard cell is a robust signalling system that is able to adapt or compensate for the chronic loss of PI-PLC, but which is unable to adjust quickly to acute loss of this component. It would be interesting to investigate this possibility further using either transient manipulation of gene expression or through the use of an inducible promoter.

Key words: Abscisic acid, calcium ions, guard cell, phospholipase C, signalling, stomata.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
P. E. Dowd, S. Coursol, A. L. Skirpan, T.-h. Kao, and S. Gilroy
Petunia Phospholipase C1 Is Involved in Pollen Tube Growth
PLANT CELL, June 1, 2006; 18(6): 1438 - 1453.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y.-Y. Pan, X. Wang, L.-G. Ma, and D.-Y. Sun
Characterization of Phosphatidylinositol-Specific Phospholipase C (PI-PLC) from Lilium daviddi Pollen
Plant Cell Physiol., October 1, 2005; 46(10): 1657 - 1665.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.