JXB Advance Access originally published online on December 12, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal of Experimental Botany, Vol. 55, No. 395, pp. 237-245, January 1, 2004
© 2004 Oxford University Press
Signalling in Abiotic Stress |
Maintenance of shoot growth by endogenous ABA: genetic assessment of the involvement of ethylene suppression
Received 17 July 2003; Accepted 14 October 2003
Department of Agronomy, Plant Sciences Unit, 1–87 Agriculture Building, University of Missouri, Columbia, MO 65211, USA
1 To whom correspondence should be addressed. Fax: +1 573 882 1469. E-mail: SharpR{at}missouri.edu
Previous work demonstrated that normal levels of endogenous abscisic acid (ABA) are required to maintain shoot growth in well-watered tomato plants independently of effects of hormone status on plant water balance. The results suggested that the impairment of shoot growth in ABA-deficient mutants is at least partly attributable to increased ethylene production. To assess the extent to which ABA maintains shoot growth by ethylene suppression, the growth of ABA-deficient (aba2-1) and ethylene-insensitive (etr1-1) single- and double-mutants of Arabidopsis was examined. To ensure that the results were independent of effects of hormone status on plant water balance, differential relative humidity regimes were used to achieve similar leaf water potentials in all genotypes and treatments. In aba2-1, shoot growth was substantially inhibited and ethylene evolution was doubled compared with the wild type, consistent with the results for tomato. In the aba2-1 etr1-1 double mutant, in which ABA was equally as deficient as in aba2-1 and shoot growth was shown to be insensitive to ethylene, shoot growth was substantially, although incompletely, restored relative to etr1-1. Treatment with ABA resulted in the complete recovery of shoot growth in aba2-1 relative to the wild type, and also significantly increased the growth of aba2-1 etr1-1 such that total leaf area and shoot fresh weight were not significantly lower than in etr1-1. In addition, ABA treatment of aba2-1 etr1-1 restored the wider leaf morphology phenotype exhibited by etr1-1. The results demonstrate that normal levels of endogenous ABA maintain shoot development, particularly leaf expansion, in well-watered Arabidopsis plants, partly by suppressing ethylene synthesis and partly by another mechanism that is independent of ethylene.
Key words: ABA, aba2-1, abscisic acid, Arabidopsis thaliana, ethylene, etr1-1, shoot growth.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. van Zanten, L. A.C.J. Voesenek, A. J.M. Peeters, and F. F. Millenaar Hormone- and Light-Mediated Regulation of Heat-Induced Differential Petiole Growth in Arabidopsis Plant Physiology, November 1, 2009; 151(3): 1446 - 1458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. C. Dodd, J. C. Theobald, S. K. Richer, and W. J. Davies Partial phenotypic reversion of ABA-deficient flacca tomato (Solanum lycopersicum) scions by a wild-type rootstock: normalizing shoot ethylene relations promotes leaf area but does not diminish whole plant transpiration rate J. Exp. Bot., October 1, 2009; 60(14): 4029 - 4039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rodrigues, J. Santiago, S. Rubio, A. Saez, K. S. Osmont, J. Gadea, C. S. Hardtke, and P. L. Rodriguez The Short-Rooted Phenotype of the brevis radix Mutant Partly Reflects Root Abscisic Acid Hypersensitivity Plant Physiology, April 1, 2009; 149(4): 1917 - 1928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ma, J. Xue, Y. Li, X. Liu, F. Dai, W. Jia, Y. Luo, and J. Gao Rh-PIP2;1, a Rose Aquaporin Gene, Is Involved in Ethylene-Regulated Petal Expansion Plant Physiology, October 1, 2008; 148(2): 894 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Duan, Y. Yang, Y. Lu, H. Korpelainen, F. Berninger, and C. Li Interactions between water deficit, ABA, and provenances in Picea asperata J. Exp. Bot., August 1, 2007; 58(11): 3025 - 3036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tholen, T. L. Pons, L. A.C.J. Voesenek, and H. Poorter Ethylene Insensitivity Results in Down-Regulation of Rubisco Expression and Photosynthetic Capacity in Tobacco Plant Physiology, July 1, 2007; 144(3): 1305 - 1315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Thompson, J. Andrews, B. J. Mulholland, J. M.T. McKee, H. W. Hilton, J. S. Horridge, G. D. Farquhar, R. C. Smeeton, I. R.A. Smillie, C. R. Black, et al. Overproduction of Abscisic Acid in Tomato Increases Transpiration Efficiency and Root Hydraulic Conductivity and Influences Leaf Expansion Plant Physiology, April 1, 2007; 143(4): 1905 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Benschop, F. F. Millenaar, M. E. Smeets, M. van Zanten, L. A.C.J. Voesenek, and A. J.M. Peeters Abscisic Acid Antagonizes Ethylene-Induced Hyponastic Growth in Arabidopsis Plant Physiology, February 1, 2007; 143(2): 1013 - 1023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rosado, I. Amaya, V. Valpuesta, J. Cuartero, M. A. Botella, and O. Borsani ABA- and ethylene-mediated responses in osmotically stressed tomato are regulated by the TSS2 and TOS1 loci J. Exp. Bot., September 1, 2006; 57(12): 3327 - 3335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Munns, R. A. James, and A. Lauchli Approaches to increasing the salt tolerance of wheat and other cereals J. Exp. Bot., March 1, 2006; 57(5): 1025 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, J. Zhang, Z. Wang, K. Liu, and P. Wang Post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene J. Exp. Bot., January 1, 2006; 57(1): 149 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Barrero, P. Piqueras, M. Gonzalez-Guzman, R. Serrano, P. L. Rodriguez, M. R. Ponce, and J. L. Micol A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development J. Exp. Bot., August 1, 2005; 56(418): 2071 - 2083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kozuka, G. Horiguchi, G.-T. Kim, M. Ohgishi, T. Sakai, and H. Tsukaya The Different Growth Responses of the Arabidopsis thaliana Leaf Blade and the Petiole during Shade Avoidance are Regulated by Photoreceptors and Sugar Plant Cell Physiol., January 15, 2005; 46(1): 213 - 223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Anderson, E. Badruzsaufari, P. M. Schenk, J. M. Manners, O. J. Desmond, C. Ehlert, D. J. Maclean, P. R. Ebert, and K. Kazan Antagonistic Interaction between Abscisic Acid and Jasmonate-Ethylene Signaling Pathways Modulates Defense Gene Expression and Disease Resistance in Arabidopsis PLANT CELL, December 1, 2004; 16(12): 3460 - 3479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Sharp, V. Poroyko, L. G. Hejlek, W. G. Spollen, G. K. Springer, H. J. Bohnert, and H. T. Nguyen Root growth maintenance during water deficits: physiology to functional genomics J. Exp. Bot., November 1, 2004; 55(407): 2343 - 2351. [Abstract] [Full Text] [PDF]
|
|