Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gallagher, J. A.
Right arrow Articles by Pollock, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gallagher, J. A.
Right arrow Articles by Pollock, C. J.
Agricola
Right arrow Articles by Gallagher, J. A.
Right arrow Articles by Pollock, C. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 397, pp. 557-569, March 1, 2004
© 2004 Oxford University Press

Cell and Molecular Biology, Biochemistry and Molecular Physiology

Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L.

Received 30 July 2003; Accepted 4 November 2003

J. A. Gallagher*, A. J. Cairns and C. J. Pollock

Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK

* To whom correspondence should be addressed. Fax: +44 (0) 1970 823243. E-mail: joe.gallagher{at}bbsrc.ac.uk

The invertases of Lolium temulentum have been characterized at the enzyme level. However, studies on the expression of the genes coding for these enzymes have been lacking. To elucidate the role of acid ‘invertase-like’ genes in sucrose metabolism and carbon partitioning in Gramineae further, three ‘invertase-like’ homologous clones were isolated from L. temulentum cDNA expression libraries based on leaf tissue, using maize soluble invertase probes. The effect of developmental stage and alterations in carbohydrate status on the expression and tissue distribution of these genes was investigated. The three highly homologous genes (Inv 1:2, Inv 1:4, and FT 2:2) show different patterns of expression and different tissue distribution. Inv 1:2 was predominantly expressed in root tissue. Expression increased during the dark in root and tiller base tissue. Minimal variations in gene expression were observed in leaf tissue following changes in carbohydrate status. Inv 1:4 was predominantly expressed in tiller bases, leaf sheath, and leaf base, with increased expression in tissue samples in the dark period. FT 2:2 was also predominantly expressed in tiller bases, leaf sheath, and leaf base. Higher expression was observed in leaf tissue following increases in carbohydrate content, in a manner that paralleled the regulation and spatial occurrence of fructan in the leaf tissue. Whilst invertases and fructosyltransferases are difficult to distinguish at the level of the whole sequence, analysis of 5' sequence and specific amino acids allows discrimination which correlates with patterns of expression within the tissue. Based on expression patterns and sequence characteristics, it is proposed that Inv 1:2 and Inv 1:4 code for soluble acid invertases, whilst FT 2:2 codes for a fructosyltransferase.

Key words: cDNA, fructan, fructosyltransferase, gene expression, invertase, sucrose.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
K.-i. Tamura, A. Kawakami, Y. Sanada, K. Tase, T. Komatsu, and M. Yoshida
Cloning and functional analysis of a fructosyltransferase cDNA for synthesis of highly polymerized levans in timothy (Phleum pratense L.)
J. Exp. Bot., March 1, 2009; 60(3): 893 - 905.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
F. del Viso, A. F. Puebla, C. M. Fusari, A. C. Casabuono, A. S. Couto, H. G. Pontis, H. E. Hopp, and R. A. Heinz
Molecular Characterization of a Putative Sucrose:Fructan 6-Fructosyltransferase (6-SFT) of the Cold-Resistant Patagonian Grass Bromus pictus Associated With Fructan Accumulation Under Low Temperatures
Plant Cell Physiol., March 1, 2009; 50(3): 489 - 503.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. Skot, J. Humphreys, M. O. Humphreys, D. Thorogood, J. Gallagher, R. Sanderson, I. P. Armstead, and I. D. Thomas
Association of Candidate Genes With Flowering Time and Water-Soluble Carbohydrate Content in Lolium perenne (L.)
Genetics, September 1, 2007; 177(1): 535 - 547.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Lasseur, J. Lothier, A. Djoumad, B. De Coninck, S. Smeekens, A. Van Laere, A. Morvan-Bertrand, W. Van den Ende, and M.-P. Prud'homme
Molecular and functional characterization of a cDNA encoding fructan:fructan 6G-fructosyltransferase (6G-FFT)/fructan:fructan 1-fructosyltransferase (1-FFT) from perennial ryegrass (Lolium perenne L.)
J. Exp. Bot., August 1, 2006; 57(11): 2719 - 2734.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.