JXB Advance Access originally published online on February 13, 2004
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Journal of Experimental Botany, Vol. 55, No. 397, pp. 771-781, March 1, 2004
© 2004 Oxford University Press
Plants and the Environment |
Mechanisms underlying the amelioration of O3-induced damage by elevated atmospheric concentrations of CO2
Received 25 November 2003; Accepted 28 November 2003
1 Museu, Laboratório e Jardim Botânico, Universidade de Lisboa, Rua da Escola Politécnica 58, 1250-102 Lisboa, Portugal
2 Departamento de Biología Vegetal I, Facultad de Ciencias Biológicas, Universidad Complutense, 28040 Madrid, Spain
3 Institute for Water and Environmental Resource Management, Dunbar Building, University of Technology, Sydney, CNR. Westbourne Street and Pacific Highway, St. Leonards, NSW 2065, Australia
4 Environmental and Molecular Plant Physiology, Institute for Research on the Environment and Sustainability [IRES], School of Biology, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK
* To whom correspondence should be addressed. Fax: +44 (0)191 222 5229. E-mail: J.D.Barnes{at}ncl.ac.uk
Abbreviations: 
app, apparent quantum yield of CO2 assimilation; A, rate of CO2 assimilation; A350, light-saturated rate of CO2 assimilation rate at an ambient CO2 concentration of 350 µl l–1; Amax, light- and CO2-saturated rate of CO2 assimilation; CFA, charcoal/Purafil®-filtered air; ci, intercellular CO2 concentration; DALE, days after leaf emergence; DAT, days after transfer; Fv/Fm, maximum quantum yield of PSII photochemistry; gH2O, stomatal conductance to water vapour; K, root/shoot allometric coefficient; RGR, plant relative growth rate; Vcmax, maximum in vivo rate of Rubisco carboxylation.
There is growing evidence that rising atmospheric CO2 concentrations will reduce or prevent reductions in the growth and productivity of C3 crops attributable to ozone (O3) pollution. In this study, the role of pollutant exclusion in mediating this response was investigated through growth chamber-based investigations on leaves 4 and 7 of spring wheat (Triticum aestivum cv. Hanno). In the core experiments, plants were raised at two atmospheric CO2 concentrations (ambient [350 µl l–1] or elevated CO2 [700 µl l–1] under two O3 regimes (charcoal/Purafil®-filtered air [<5 nl l–1 O3] or ozone-enriched air [75 nl l–1 7 h d–1]). A subsequent experiment used an additional O3 treatment where the goal was to achieve equivalent daily O3 uptake over the life-span of leaves 4 and 7 under ambient and CO2-enriched conditions, through daily adjustment of exposures based on measured shifts in stomatal conductance. Plant growth and net CO2 assimilation were stimulated by CO2-enrichment and reduced by exposure to O3. However, the impacts of O3 decreased with plant age (i.e. leaf 7 was more resistant to O3 injury than leaf 4); a finding consistent with ontogenic shifts in the tolerance of plant tissue and/or acclimation to O3-induced oxidative stress. In the combined treatment, elevated CO2 protected against the adverse effects of O3 and reduced cumulative O3 uptake (calculated from measurements of stomatal conductance) by c. 10% and 35% over the life-span of leaves 4 and 7, respectively. Analysis of the relationship between O3 uptake and the decline in the maximum in vivo rate of Rubisco carboxylation (Vcmax) revealed the protection afforded by CO2-enrichment to be due, to a large extent, to the exclusion of the pollutant from the leaf interior (as a consequence of the decline in stomatal conductance triggered by CO2-enrichment), but there was evidence (especially from flux–response relationships constructed for leaf 4) that CO2-enrichment resulted in additional effects that alleviated the impacts of ozone-induced oxidative stress on photosynthesis.
Key words: Air pollutant interactions, detoxification, ozone uptake, rising atmospheric CO2 concentrations, spring wheat.
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