Skip Navigation


JXB Advance Access originally published online on May 7, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/400/1221    most recent
erh143v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (32)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lloyd, J. C.
Right arrow Articles by Zakhleniuk, O. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lloyd, J. C.
Right arrow Articles by Zakhleniuk, O. V.
Agricola
Right arrow Articles by Lloyd, J. C.
Right arrow Articles by Zakhleniuk, O. V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 400, pp. 1221-1230, May 1, 2004
© 2004 Oxford University Press

Photosynthetic Gene Expression

Responses of primary and secondary metabolism to sugar accumulation revealed by microarray expression analysis of the Arabidopsis mutant, pho3

Received 26 November 2003; Accepted 4 March 2004

Julie C. Lloyd* and Oksana V. Zakhleniuk

Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK

* To whom correspondence should be addressed. Fax: +44 1206 872592. E-mail: lloyj{at}essex.ac.uk

The Arabidopsis mutant pho3 accumulates sucrose and other carbohydrates to high levels, providing a means of investigating the genomic response to sucrose accumulation using microarray analysis. Wild-type and mutant plants were grown in soil to the mature rosette stage for the analysis of gene expression using the Affymetrix ATH1 chip, containing more than 22 500 probe sets. Small, but significant, decreases were observed in the expression of many genes encoding enzymes and regulatory proteins involved in primary carbon assimilation, suggesting that, in mature leaves of Arabidopsis, there is limited feedback regulation on gene expression by sugars. The study revealed a striking increase in the expression of the plastid glucose 6-phosphate/phosphate translocator, characteristically expressed only in heterotrophic tissues. This indicated a change in the nature of metabolite exchange between the plastid and the cytosol in the pho3 mutant. The expression of enzymes of starch synthesis also increased significantly. Very large increases were observed in the expression of transcription factors and enzymes involved in anthocyanin biosynthesis. This finding reinforces the emerging picture of an important role for primary metabolism in regulating secondary metabolism.

Key words: Anthocyanin, Arabidopsis thaliana, GPT, MYB proteins, primary metabolism, suc2, sucrose, sugar signalling.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
K. Zhou, M. Yamagishi, M. Osaki, and K. Masuda
Sugar signalling mediates cluster root formation and phosphorus starvation-induced gene expression in white lupin
J. Exp. Bot., July 1, 2008; 59(10): 2749 - 2756.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. P. Hammond and P. J. White
Sucrose transport in the phloem: integrating root responses to phosphorus starvation
J. Exp. Bot., January 1, 2008; 59(1): 93 - 109.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. F. Baker and D. M. Braun
tie-dyed1 Functions Non-Cell Autonomously to Control Carbohydrate Accumulation in Maize Leaves
Plant Physiology, June 1, 2007; 144(2): 867 - 878.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
U. Richter, U. Sonnewald, and B. Drager
Calystegines in potatoes with genetically engineered carbohydrate metabolism
J. Exp. Bot., May 1, 2007; 58(7): 1603 - 1615.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Carrera, T. Holman, A. Medhurst, W. Peer, H. Schmuths, S. Footitt, F. L. Theodoulou, and M. J. Holdsworth
Gene Expression Profiling Reveals Defined Functions of the ATP-Binding Cassette Transporter COMATOSE Late in Phase II of Germination
Plant Physiology, April 1, 2007; 143(4): 1669 - 1679.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Muller, M. Morant, H. Jarmer, L. Nilsson, and T. H. Nielsen
Genome-Wide Analysis of the Arabidopsis Leaf Transcriptome Reveals Interaction of Phosphate and Sugar Metabolism
Plant Physiology, January 1, 2007; 143(1): 156 - 171.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Bari, B. Datt Pant, M. Stitt, and W.-R. Scheible
PHO2, MicroRNA399, and PHR1 Define a Phosphate-Signaling Pathway in Plants
Plant Physiology, July 1, 2006; 141(3): 988 - 999.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Nicolai, M.A. Roncato, A.S. Canoy, D. Rouquie, X. Sarda, G. Freyssinet, and C. Robaglia
Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control
Plant Physiology, June 1, 2006; 141(2): 663 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Solfanelli, A. Poggi, E. Loreti, A. Alpi, and P. Perata
Sucrose-Specific Induction of the Anthocyanin Biosynthetic Pathway in Arabidopsis
Plant Physiology, February 1, 2006; 140(2): 637 - 646.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Teng, J. Keurentjes, L. Bentsink, M. Koornneef, and S. Smeekens
Sucrose-Specific Induction of Anthocyanin Biosynthesis in Arabidopsis Requires the MYB75/PAP1 Gene
Plant Physiology, December 1, 2005; 139(4): 1840 - 1852.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
G. J. G. Upton and J. C. Lloyd
Oligonucleotide arrays: information from replication and spatial structure
Bioinformatics, November 15, 2005; 21(22): 4162 - 4168.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Usadel, A. Nagel, O. Thimm, H. Redestig, O. E. Blaesing, N. Palacios-Rojas, J. Selbig, J. Hannemann, M. C. Piques, D. Steinhauser, et al.
Extension of the Visualization Tool MapMan to Allow Statistical Analysis of Arrays, Display of Coresponding Genes, and Comparison with Known Responses
Plant Physiology, July 1, 2005; 138(3): 1195 - 1204.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.