Skip Navigation


JXB Advance Access originally published online on July 2, 2004
Journal of Experimental Botany 2004 55(403):1623-1633; doi:10.1093/jxb/erh186
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/403/1623    most recent
erh186v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Pateraki, I.
Right arrow Articles by Kanellis, A. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pateraki, I.
Right arrow Articles by Kanellis, A. K.
Agricola
Right arrow Articles by Pateraki, I.
Right arrow Articles by Kanellis, A. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 55, No. 403, © Society for Experimental Biology 2004; all rights reserved

RESEARCH PAPER

Molecular characterization and expression studies during melon fruit development and ripening of L-galactono-1,4-lactone dehydrogenase

Irene Pateraki1,2, Maite Sanmartin1,2,3 *, Mary S. Kalamaki1, Dimitrios Gerasopoulos1 {dagger} and Angelos K. Kanellis1,2,{ddagger}

1Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognocy, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece
2Institute of Viticulture and Vegetable Crops, National Agriculture Research Foundation, PO Box 2229, 713 09 Heraklion, Crete, Greece
3Institute of Molecular Biology and Biotechnology, FORTH, PO Box 1527, 711 10 Heraklion, Crete, Greece

{ddagger} To whom correspondence should be addressed. Fax: +30 2310 997662, 997645. E-mail: kanellis{at}pharm.auth.gr

The last step of ascorbic acid (AA) biosynthesis is catalysed by the enzyme L-galactono-1,4-lactone dehydrogenase (GalLDH, EC 1.3.2.3), located on the inner mitochondrial membrane. The enzyme converts L-galactono-1,4-lactone to ascorbic acid (AA). In this work, the cloning and characterization of a GalLDH full-length cDNA from melon (Cucumis melo L.) are described. Melon genomic DNA Southern analysis indicated that CmGalLDH was encoded by a single gene. CmGalLDH mRNA accumulation was detected in all tissues studied, but differentially expressed during fruit development and seed germination. It is hypothesized that induction of CmGalLDH gene expression in ripening melon fruit contributes to parallel increases in the AA content and so playing a role in the oxidative ripening process. Higher CmGalLDH message abundance in light-grown seedlings compared with those raised in the dark suggests that CmGalLDH expression is regulated by light. Finally, various stresses and growth regulators resulted in no significant change in steady state levels of CmGalLDH mRNA in 20-d-old melon seedlings. To the authors' knowledge, this is the first report of GalLDH transcript induction in seed germination and differential gene expression during fruit ripening.

Key words: Ascorbic acid, biosynthesis, Cucumis melo L., fruit development, L-galactono-1,4-lactone dehydrogenase, melon, ripening


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
S. M. Bulley, M. Rassam, D. Hoser, W. Otto, N. Schunemann, M. Wright, E. MacRae, A. Gleave, and W. Laing
Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis
J. Exp. Bot., March 1, 2009; 60(3): 765 - 778.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Ioannidi, M. S. Kalamaki, C. Engineer, I. Pateraki, D. Alexandrou, I. Mellidou, J. Giovannonni, and A. K. Kanellis
Expression profiling of ascorbic acid-related genes during tomato fruit development and ripening and in response to stress conditions
J. Exp. Bot., February 1, 2009; 60(2): 663 - 678.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Loscos, M. A. Matamoros, and M. Becana
Ascorbate and Homoglutathione Metabolism in Common Bean Nodules under Stress Conditions and during Natural Senescence
Plant Physiology, March 1, 2008; 146(3): 1282 - 1292.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. A. Wolucka, A. Goossens, and D. Inze
Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions
J. Exp. Bot., September 1, 2005; 56(419): 2527 - 2538.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.